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  1. No Access

    Protocol

    Methods for Analyzing the Gut Microbiota in Diabetic Retinopathy

    The gut microbiome that inhabits human hosts plays an important role in the development of a healthy host immune system. Many studies have shown that gut microbiota is involved in the occurrence and developmen...

    Yinhua Huang, Jacey Hongjie Ma, Yanfang Peng, Xu Yang, Jiansu Chen… in Diabetic Retinopathy (2023)

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    Protocol

    Chromatin Immunoprecipitation Assay in Mouse Retinal Tissue

    Chromatin immunoprecipitation (ChIP) is one of the most widely used methods for investigating interactions between proteins and DNA sequences. ChIP plays an important role in the transcriptional regulation stu...

    Zhijie Wang, Shangli Ji, Jacey Hongjie Ma, Jiansu Chen, Shibo Tang in Diabetic Retinopathy (2023)

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    Protocol

    Investigating the Function of MicroRNAs in Human Retinal Microvascular Endothelial Cells of Diabetic Retinopathy

    Diabetic retinopathy (DR) is the main complication of diabetes mellitus (DM). Recent studies have implicated microRNAs dysfunction in human retinal microvascular endothelial cell (HRMEC). In this study, we aim...

    Yong Zeng, Zekai Cui, Jiansu Chen, Shibo Tang in Diabetic Retinopathy (2023)

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    Protocol

    Methods for RPE Sheet Construction Using iPS-CM in Conjunction with Natural Scaffold of Corneal Lenticule Derived from SMILE

    In vitro generation of a functional retinal pigment epithelium (RPE) monolayer sheet is useful and promising for RPE cell therapy. Here, we outline a method to construct engineered RPE sheets treated by induce...

    Jianing Gu, Yini Wang, Chengcheng Ding, Shibo Tang, Jiansu Chen in Diabetic Retinopathy (2023)