81 Result(s)
within
Protocol
Jia **g
Life Sciences
-
Protocol
Co-immunoprecipitation-Based Isolation of Double-Stranded RNA-Associated Protein Complexes in Nicotiana benthamiana
Double-stranded RNA (dsRNA) is associated with most viral infections, and is generated in host cells during viral replication. Viral RNA replication occurs within the viral factories called the viral replicati...
-
Protocol
Assessing Seed Germination Response of Parasitic Plant Striga hermonthica with Small-Molecule Probes
Seed germination of a parasitic plant Striga hermonthica is elicited by strigolactones which are exuded from roots of host plants. Here, we describe a high-throughput germination assay and a method for visualizin...
-
Protocol
Use of Specific Borrelia Phages as a New Strategy for Improved Diagnostic Tests
The high failure rate of tick-borne infection (TBI)-related testing underscores the need for novel approaches that do not rely on serology and two-tier testing. Delayed diagnosis of TBIs, especially Borrelia infe...
-
Protocol
Holographic Optogenetic Activation of Neurons Eliciting Locomotion in Head-Embedded Larval Zebrafish
Understanding how motor circuits are organized and recruited in order to perform complex behavior is an essential question of neuroscience. Here we present an optogenetic protocol on larval zebrafish that allo...
-
Protocol
Pseudouridine Identification and Functional Annotation with PIANO
Pseudouridine (Ψ) is the first-discovered RNA modification abundantly present in many classes of RNAs, which plays a pivotal role in a series of biological processes. Accurately identifying the location of Ψ s...
-
Protocol
Haplotype-Assisted Noninvasive Prenatal Diagnosis of Genetic Diseases by Massively Parallel Sequencing of Maternal Plasma Cell-Free DNA
Early prenatal diagnosis of genetic diseases allows for timely intervention or prevention of the diseases in newborns. Conventional prenatal diagnosis of most genetic diseases relies on testing fetal DNA obta...
-
Protocol
Pyrophosphorylated-Cholesterol-Modified Bone-Targeting Liposome Formulation Procedure
Bone-targeting drug delivery systems have been rapidly developed to increase drug efficacy and safety for musculoskeletal diseases in the past decades. Bone-targeting drug delivery is mainly based on ligands t...
-
Protocol
Quantitative Analysis of Nucleoside Triphosphate Pools in Mouse Muscle Using Hydrophilic Interaction Liquid Chromatography Coupled with Tandem Mass Spectrometry Detection
Defects in deoxyribonucleoside triphosphate (dNTP) metabolism are associated with a number of mitochondrial DNA (mtDNA) depletion syndromes (MDS). These disorders affect the muscles, liver, and brain, and the ...
-
Protocol
Inference of Ancient Polyploidy Using Transcriptome Data
Polyploidizations, or whole-genome duplications (WGDs), in plants have increased biological complexity, facilitated evolutionary innovation, and likely enabled adaptation under harsh conditions. Besides genomi...
-
Protocol
Crystallization and Structural Determination of 8–17 DNAzyme
DNAzymes are a group of DNA molecules that can catalyze various chemical reactions. Owing to their great application potentials, DNAzymes have received significant attention. However, due to their intrinsic di...
-
Protocol
Characterization of Smoothened Phosphorylation and Activation
The GPCR-family protein Smoothened (Smo) is an obligatory signal transducer of the Hedgehog (Hh) signaling pathway. Binding of Hh to its receptor Patched (Ptc) alleviates Ptc-mediated inhibition of Smo, allowi...
-
Protocol
Induction of Human Naïve Pluripotent Stem Cells from Somatic Cells
Generating patient-specific stem cells representing the onset of development has become possible since the discovery of somatic cell reprogramming into induced pluripotent stem cells. However, human pluripoten...
-
Protocol
Dissection, Fixation, and Immunostaining of the Drosophila Midgut
The Drosophila midgut is mainly composed of highly polarized epithelial cells called enterocytes that establish their apical-basal polarity in a fundamentally different way from other Drosophila epithelia. The ro...
-
Protocol
An Automatic Stage Identification MATLAB Tool to Reveal Notch Expression Pattern in Drosophila Egg Chambers
Many highly conserved pathways control the development and determine cell fate in organisms. One of these pathways is the Notch signaling pathway that allows for local cell–cell communication. Researchers have...
-
Protocol
Methods to Quantify Cell Division and Hormone Gradients During Root Tropisms
Tropisms are growth-based plant directional movements, allowing plants to respond to their living environments. Plant roots have developed various tropic responses, including gravitropism, hydrotropism, chemot...
-
Protocol
Fabrication of a Completely Biological and Anisotropic Human Mesenchymal Stem Cell-Based Vascular Graft
Tissue-engineered small-diameter vascular grafts are required to match mechanical properties as well as cellular and extracellular architecture of native blood vessels. Although various engineering technologie...
-
Protocol
Bioinformatics Tools to Understand Notch
As a result of the culmination of data, and the fast-paced advancement of new research, all the biological information collected can make it difficult to sort data. This is oftentimes experienced when learning...
-
Protocol
Generation of Thermally Stable Affinity Pairs for Sensitive, Specific Immunoassays
Many point-of-care diagnostic tests rely on a pair of monoclonal antibodies that bind to two distinct epitopes of a molecule of interest. This protocol describes the identification and generation of such affin...
-
Protocol
Nanobodies: From Serendipitous Discovery of Heavy Chain-Only Antibodies in Camelids to a Wide Range of Useful Applications
The presence of unique heavy chain-only antibodies (HCAbs) in camelids was discovered at Vrije Universiteit Brussel (VUB, Brussels, Belgium) at a time when many researchers were exploring the cloning and expre...
-
Protocol
Cytoplasmic Expression of Nanobodies with Formylglycine Generating Enzyme Tag and Conversion to a Bio-Orthogonal Aldehyde Group
Nanobodies (Nbs) can be successfully retrieved following phage, bacterial, yeast, or ribosome display of immune, synthetic, or naïve libraries. However, after panning, multiple individual Nb clones need to be ...
