![Loading...](https://link.springer.com/static/c4a417b97a76cc2980e3c25e2271af3129e08bbe/images/pdf-preview/spacer.gif)
-
Protocol
Protoplast Electrofusion and Regeneration in Potato
Protoplast fusion and subsequent in vitro plant regeneration, leading to somatic hybridization, offer opportunities for transferring entire genomes from one plant into another, regardless of the interspecific ...
-
Protocol
MSACompro: Improving Multiple Protein Sequence Alignment by Predicted Structural Features
Multiple Sequence Alignment (MSA) is an essential tool in protein structure modeling, gene and protein function prediction, DNA motif recognition, phylogenetic analysis, and many other bioinformatics tasks. Th...
-
Protocol
The MULTICOM Protein Tertiary Structure Prediction System
With the expansion of genomics and proteomics data aided by the rapid progress of next-generation sequencing technologies, computational prediction of protein three-dimensional structure is an essential part o...
-
Protocol
Gene Delivery Method Using Photo-Responsive Poly(β-Amino Ester) as Vectors
Nonviral vectors show great potential in delivering nucleic acids (NA) into many mammalian cells to achieve efficient gene transfection. Among these, cationic polymer is one of the most widely used nonviral ge...
-
Protocol
Highly Efficient SiRNA Delivery Mediated by Cationic Helical Polypeptides and Polypeptide-Based Nanosystems
RNA interference (RNAi) mediated by small interfering RNA (SiRNA) has recently emerged as a potent machinery in regulating gene expression at the post-translation step. Despite its efficiency and specificity, ...
-
Protocol
Protein Residue Contacts and Prediction Methods
In the field of computational structural proteomics, contact predictions have shown new prospects of solving the longstanding problem of ab initio protein structure prediction. In the last few years, application ...
-
Protocol
Assessing Predicted Contacts for Building Protein Three-Dimensional Models
Recent successes of contact-guided protein structure prediction methods have revived interest in solving the long-standing problem of ab initio protein structure prediction. With homology modeling failing for ...
-
Protocol
The Gene Balance Hypothesis: Epigenetics and Dosage Effects in Plants
Dosage effects in plants are caused by changes in the copy number of chromosomes, segments of chromosomes, or multiples of individual genes. Genes often exhibit a dosage effect in which the amount of product i...
-
Protocol
The MULTICOM Protein Structure Prediction Server Empowered by Deep Learning and Contact Distance Prediction
Prediction of the three-dimensional (3D) structure of a protein from its sequence is important for studying its biological function. With the advancement in deep learning contact distance prediction and residu...
-
Protocol
Genetic Studies of Natural Glycosphingolipid Ligands for NKT Cells
Glycosphingolipids (GSL) are natural ligands of NKT cells. Several laboratories have reported the in vitro activity of isoglobotriosylceramide (iGb3) in stimulating NKT cells. However, the knockout mice of iGb...