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    Protocol

    Generation of Deletion Lines in Allohexaploid Bread Wheat

    Positional cloning in bread wheat (Triticum aestivum L.) remains a daunting task because of its large genome, high density of repeats, low recombination rate especially in pericentromeric regions and its allopoly...

    Heïdi Serra, Radim Svačina, Jan Bartoš, Pierre Sourdille in Plant Gametogenesis (2022)

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    Protocol

    Localization of Low-Copy DNA Sequences on Mitotic Chromosomes by FISH

    Fluorescence in situ hybridization (FISH) is a widely used method to localize DNA sequences on mitotic and meiotic chromosomes and interphase nuclei. It was developed in early 1980s and since then it has contr...

    Miroslava Karafiátová, Jan Bartoš, Jaroslav Doležel in Plant Cytogenetics (2016)