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Protocol
Generation of Deletion Lines in Allohexaploid Bread Wheat
Positional cloning in bread wheat (Triticum aestivum L.) remains a daunting task because of its large genome, high density of repeats, low recombination rate especially in pericentromeric regions and its allopoly...
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Protocol
Localization of Low-Copy DNA Sequences on Mitotic Chromosomes by FISH
Fluorescence in situ hybridization (FISH) is a widely used method to localize DNA sequences on mitotic and meiotic chromosomes and interphase nuclei. It was developed in early 1980s and since then it has contr...