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Protocol
Remote Ischemic Pre-Conditioning (RIPC)
Ischemic pre-conditioning has been shown to protect hearts against ischemia/reperfusion (I/R)-induced cardiac injury. However, it is not feasible in clinic. Many researchers have tried to introduce brief I/R i...
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Protocol
A Surgical Approach to Hindlimb Suspension: A Mouse Model of Disuse-Induced Atrophy
Hindlimb suspension is a well-established rodent model of disuse-induced atrophy and is commonly used to simulate the effects of bed rest and space flight on humans. Over the decades, this method has undergone...
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Protocol
Mouse Corneal Transplantation
Corneal transplantation is the most common form of organ transplantation worldwide. Transplant survival depends on various factors, many of which are not fully understood. Due to the existence of many genetica...
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Protocol
Detecting m6A with In Vitro DART-Seq
Recent studies have uncovered that cellular mRNAs contain a diverse epitranscriptome comprising chemically modified bases which play important roles in gene expression regulation. Among these is m6A, which is a h...
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Protocol
In Vitro Stimulation of IRE1α/XBP1-Deficient B Cells with LPS
During immune responses, pathogen-specific B cells differentiate into plasma cells. Plasma cells synthesize and secrete large amounts of immunoglobulin (Ig) molecules which play a central role in immunity agai...
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Protocol
Phylogenetic and Recombination Analysis of Animal Coronaviruses
Coronavirus (CoV) is a highly diverse virus family with a broad host range and geographic distribution. When spillover occurs, sometimes it may cause major disease outbreaks in the new host or in a new territo...
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Protocol
Role of G-Protein Coupled Receptors in Chemotaxis of Innate Lymphoid Cells
Innate lymphoid cells (ILCs) are a recently identified family of immune cells mostly present at barrier surfaces. They play an important role in the induction, regulation, and resolution of inflammatory respon...
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Protocol
An Improved Method to Produce Clinical-Scale Natural Killer Cells from Human Pluripotent Stem Cells
Human natural killer (NK) cell-based adoptive anticancer immunotherapy has gained intense interest with many clinical trials actively recruiting patients to treat a variety of both hematological malignancies a...
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Protocol
An Efficient Protocol for VZV BAC-Based Mutagenesis
Varicella-zoster virus (VZV) causes both varicella (chicken pox) and herpes zoster (shingles). As a member of the human herpesvirus family, VZV contains a large 125-kb DNA genome, encoding 70 unique open readi...
