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    Protocol

    Organ Bioluminescence Imaging Under Machine Perfusion Setting for Assessing Quality of Harvested Organ Preservation

    Determination of organ viability over a period of time is a key technology in the process of organ preservation. However, a robust methodology to address this issue has not been established. Luciferase-express...

    Yuhei Higashi, Jun Homma, Hidekazu Sekine in Bioluminescence (2022)

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    Protocol

    Tubular Cardiac Tissue Bioengineered from Multi-Layered Cell Sheets for Use in the Treatment of Heart Failure

    This chapter describes a method for creating tubular cardiac tissue in vitro. Thick cardiac tissue in a tubular configuration is prepared by stacking cell sheets stepwise on the inner wall of a segment of smal...

    Hidekazu Sekine, Teruo Okano in Cardiac Tissue Engineering (2022)

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    Protocol

    Cell Sheet Technology for Cardiac Tissue Engineering

    In this chapter, we describe the methods for the fabrication and transfer/transplantation of 3D tissues by using cell sheet technology for cardiac tissue regeneration. A temperature-responsive culture surface ...

    Yuji Haraguchi, Tatsuya Shimizu, Katsuhisa Matsuura… in Cardiac Tissue Engineering (2014)