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Protocol
Technologies for Live Imaging of Enteric Neural Crest-Derived Cells
Time-lapse imaging of gut explants from embryonic mice in which neural crest-derived cells express fluorescent proteins allows the behavior of enteric neural crest cells to be observed and analyzed. Explants o...
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Protocol
Generation of Neural Crest Progenitors from Human Pluripotent Stem Cells
There are a vast range of diseases and disorders that are neurocristopathic in origin, including Hirschsprung’s disease, pheochromocytoma, familial dysautonomia, craniofacial disorders, and melanomas. Having a...
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Protocol
Addressing Interdisciplinary Difficulties in Developmental Biology/Mathematical Collaborations: A Neural Crest Example
Mathematical modeling can allow insight into the biological processes that can be difficult to access by conventional biological means alone. Such projects are becoming increasingly attractive with the appeara...
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Protocol
Neural Crest Cell Outgrowth Cultures and the Analysis of Cell Migration
The neural crest gives rise to many differentiated cell types including craniofacial connective tissue, peripheral neurons, Schwann cells, various endocrine organs, and pigment cells and it has been extensivel...