-
Protocol
Plasmon-Enhanced Characterization of Single Extracellular Vesicles
Extracellular vesicles (EVs) represent heterogeneous populations of membrane-bound vesicles shed from almost all kinds of cells. Although superior to conventional methods, most newly developed EV sensing platf...
-
Protocol
Use of CIDEA Reporter Mouse Model for Screening Thermogenic Fat-Activating Drugs
Excessive fat accumulation is a risk factor for metabolic diseases. Activating non-shivering thermogenesis in adipose tissue increases energy expenditure and potentially reverses obesity-related metabolic dysf...
-
Protocol
Measurement of Molecular Height Using Cell Surface Optical Profilometry (CSOP)
The plasma membrane of cells is covered by proteins, glycoproteins, and glycolipids with molecular heights ranging from just a few nanometers to hundreds of nanometers. Formation of cell-cell contacts and sign...
-
Protocol
Recent Advances in Computational Modeling of Multi-targeting Inhibitors as Anti-Alzheimer Agents
Alzheimer’s disease (AD) is a neurodegenerative illness that affects the brain and is linked to cognitive decline, memory problems, and behavioral changes. It is highly prevalent in the elderly, with a constan...
-
Protocol
Umbrella Sampling-Based Method to Compute Ligand-Binding Affinity
Many proteins have a solvent-exposed binding cleft, which permits their inhibitors to bind and unbind without significant protein conformation transforms. The binding/unbinding pathways of these protein–inhibi...
-
Protocol
Functionally Active Eosinophil Purification from Peripheral Blood
The choice of isolation technique for human peripheral blood eosinophils contributes to the understanding of clinically relevant data derived from in vitro research. Since the 1990s, eosinophils have been conv...
-
Protocol
Detection of lncRNA by LNA-Based In Situ Hybridization in Paraffin-Embedded Cancer Cell Spheroids
Cancer cell spheroids are considered important preclinical tools to evaluate the efficacy of new drugs. In cancer cell spheroids, the cells assemble and grow in 3D structures with cell contact interactions tha...
-
Protocol
A Rodent Model of Hypertrophic Scarring: Splinting of Rat Wounds
Human hypertrophic scars are the result of imperfect healing of skin, which is particularly evident from the scars develo** after severe burns. In contrast, mouse and rat full-thickness skin wounds heal norm...
-
Protocol
Myofibroblast Markers and Microscopy Detection Methods in Cell Culture and Histology
The identification of myofibroblasts is essential for mechanistic in vitro studies, cell-based drug tests, and to assess the level of fibrosis in experimental animal or human fibrosis. The name myo-fibroblast ...
-
Protocol
Using Bioengineered Fluorescence for Selective In Vivo and Ex Vivo Tracking of Intestinal Organoids Derived from Human Pluripotent Stem Cells
For current and future applications of human intestinal organoids (hIOs) to various aspects of in vivo research and their potential clinical use, an efficient noninvasive system is needed to directly visualize...
-
Protocol
CRISPR/Cas9-Based Gene Editing in Soybean
CRISPR/Cas9 (clustered regularly interspaced short palindromic repeat/CRISPR associated Cas9)-based gene editing is a robust tool for functional genomics research and breeding programs in various crops. In soy...
-
Protocol
Programmable Chromosome Painting with Oligopaints
Current methods for chromosome painting via fluorescence in situ hybridization (FISH) are costly, time-consuming, and limited in complexity. In contrast to conventional sources of probe, Oligopaints are comput...
-
Protocol
Assessment of ESCRT Protein CHMP5 Activity on Client Protein Ubiquitination by Immunoprecipitation and Western Blotting
The charged multivesicular body protein-5 (CHMP5) is a member of the endosomal-sorting complex required for transport (ESCRT) that controls membrane-scission events in eukaryotic cells. Recent studies have rev...
-
Protocol
Genotypic and Phenotypic Assays to Distinguish Vibrio cholerae Biotype
Vibrio cholerae is a motile gram-negative bacterium found in brackish water and the etiological agent of the fecal-oral disease cholera. Classical and El Tor are two main biotypes that make up the V. cholerae O1...
-
Protocol
Isolation of Mouse Primary Gastric Epithelial Cells to Investigate the Mechanisms of Helicobacter pylori Associated Disease
The gastrointestinal epithelium provides the first line of defense against invading pathogens, among which Helicobacter pylori is linked to numerous gastric pathologies, including chronic gastritis and cancer. Pr...
-
Protocol
Laboratory Culturing Techniques and Maintenance of Vibrio cholerae
Cholera is a severe diarrheal disease caused by the consumption of food or water contaminated with the aquatic gram-negative bacterium Vibrio cholerae. Infected hosts will experience vomiting and severe watery di...
-
Protocol
Recent Advances and Clinical Applications of Exon Inclusion for Spinal Muscular Atrophy
Spinal muscular atrophy (SMA) is an autosomal recessive disorder caused by a mutation in SMN1 that stops production of SMN (survival of motor neuron) protein. Insufficient levels of SMN results in the loss of mot...
-
Protocol
Effects of Ethylene on Seed Germination of Halophyte Plants Under Salt Stress
Halophyte plant species are those that can finish their life cycle in the presence of 50% or more seawater concentration. Ethylene, as a natural plant hormone produced at later stages of seed germination, play...
-
Protocol
Ultra-Sensitive Surface Plasmon Resonance Detection by Colocalized 3D Plasmonic Nanogap Arrays
Ultra-sensitive detection based on surface plasmon resonance (SPR) was investigated using 3D nanogap arrays for colocalization of target molecular distribution and localized plasmon wave in the near-field. Col...
-
Protocol
Quantum Dot-Based In Situ Hybridization and Immunohistochemistry to Detect mRNA and Protein at Subcellular Levels, Comparison with Studies Using Electron Microscopy
Semiconductor nanocrystals (Quantum dots, Qdots) have advantages of narrow, symmetric emission spectra with multiple resolvable colors that can be excited simultaneously using a single excitation wavelength. T...
