315 Result(s)
-
Protocol
Nanoliter-Scale Sample Preparation for Single-Cell Proteomic Analysis Using Glass-Oil-Air-Droplet Chip
Single-cell proteomic analyses are of fundamental importance in order to capture biological heterogeneity within complex cell systems’ heterogeneous populations. Mass spectrometry (MS)-based proteomics is a pr...
-
Protocol
Measuring Canopy Gas Exchange Using CAnopy Photosynthesis and Transpiration Systems (CAPTS)
Canopy photosynthesis (Ac), rather than leaf photosynthesis, is critical to gaining higher biomass production in the field because the daily or seasonal integrals of Ac correlate with the daily or seasonal integr...
-
Protocol
A Guide to Quantify Arabidopsis Seedling Thermomorphogenesis at Single Timepoints and by Interval Monitoring
Temperature-induced elongation of hypocotyls, petioles, and , together with hyponastic leaf responses, constitute key model phenotypes that can be used to assess a plant’s capacity for thermomorphogenesis. Ph...
-
Protocol
Expression and Purification of Mammalian NMDA Receptor Protein for Functional Characterization
N-methyl-D-aspartate (NMDA) receptors are critical for brain function and serve as drug targets for the treatment of neurological and psychiatric disorders. They typically form the tetrameric assembly of GluN1-.....
-
Protocol
Design and Construction of Antibody Fusion Proteins Incorporating Variable New Antigen Receptor (VNAR) Domains
Antibody therapeutics have become a cornerstone of the pharmaceutical market due to their precise molecular targeting, favorable pharmacokinetic properties, and multitiered mechanisms of action. Since the firs...
-
Protocol
MicroRNA Detection with CRISPR/Cas
Low-cost detection of miRNAs has caught broad attention in recent years due to the potential application of these small noncoding RNAs for diagnostics and therapeutic purposes. Their small size and low abundan...
-
Protocol
Visualization of mtDNA Using FISH
Proper mitochondrial DNA (mtDNA) levels are critical for many cellular biological functions and are associated with aging and many mitochondria disorders. Defects in core subunits of the mtDNA replication mach...
-
Protocol
Scalable Liposome Synthesis by High Aspect Ratio Microfluidic Flow Focusing
Microfluidic flow focusing provides an efficient approach to the generation of nanoscale lipid vesicles of tunable size and low size variance. Scalable nanoliposome synthesis over a wide range of production ra...
-
Protocol
Remote Ischemic Pre-Conditioning (RIPC)
Ischemic pre-conditioning has been shown to protect hearts against ischemia/reperfusion (I/R)-induced cardiac injury. However, it is not feasible in clinic. Many researchers have tried to introduce brief I/R i...
-
Protocol
Unbiased Quantification of Golgi Scattering and Golgi–Centrosome Association
The vertebrate Golgi complex is a large dynamic organelle which undergoes morphological changes and fragmentation both as a part of normal physiological dynamics and under disease conditions. The Golgi is know...
-
Protocol
Investigation of ROP GTPase Activity and Cytoskeleton Dynamics During Tip Growth in Root Hairs and Pollen Tubes
Pollen tubes and root hairs are typical tip-growing cells and are employed as model systems to study plant cell polarity. Previous studies have shown that the Rho family ROP GTPase plays a critical role in the...
-
Protocol
Role of Circulating Tumor DNA in Colorectal Cancer
Colorectal cancer (CRC) is a very common gastrointestinal tumor, ranking second in the global cause of cancer death. Because of the invasive nature of biopsy and cannot reflect the heterogeneity of tumor or mo...
-
Protocol
High-Throughput Separation and Enrichment of Rare Malignant Tumor Cells from Large-Volume Effusions by Inertial Microfluidics
Detection of malignant tumor cells (MTCs) in pleural effusions is essential for determining the malignancy. However, the sensitivity of MTC detection is significantly decreased due to the existence of a massiv...
-
Protocol
A Surgical Approach to Hindlimb Suspension: A Mouse Model of Disuse-Induced Atrophy
Hindlimb suspension is a well-established rodent model of disuse-induced atrophy and is commonly used to simulate the effects of bed rest and space flight on humans. Over the decades, this method has undergone...
-
Protocol
Targeted Absolute Protein Quantification Using SILAC Internal Standard and Full-Length Protein Calibrators (TAQSI)
Mass spectrometry (MS)-based proteomics has been increasingly used for targeted absolute protein quantifications in both basic and clinical research. There is a great need to overcome some pitfalls of current ...
-
Protocol
Imaging Brown Adipose Tissue with TSPO PET Tracers in Preclinical Animal Studies
Brown adipose tissue (BAT) is closely associated with thermogenesis and related to numerous diseases, including type 2 diabetes, nonalcoholic fatty liver disease (NAFLD), and obesity. Using molecular imaging t...
-
Protocol
Imaging of Cortical Microtubules in Plants Under Salt Stress
The microtubule (MT) network is a highly dynamic subcellular structure playing an important role in the growth and development of plants, and it is able to respond to biotic and abiotic environmental signals. ...
-
Protocol
Mouse Corneal Transplantation
Corneal transplantation is the most common form of organ transplantation worldwide. Transplant survival depends on various factors, many of which are not fully understood. Due to the existence of many genetica...
-
Protocol
Assessing SIRT7 Activity In Vivo and In Vitro in Response to DNA Damage
The class III histone deacetylase (HDACs) also known as sirtuins (SIRTs 1–7) are ubiquitously expressed, but SIRT7 mainly resides as nucleolar protein. In this chapter a couple of methods are described that ar...
-
Protocol
Methods to Visualize and Quantify Cortical Microtubule Arrays in Arabidopsis Conical Cells
Many studies from different model organisms have demonstrated that microtubules are essential for various cellular processes, including cell division, cell morphogenesis, and intracellular trafficking. In inte...
