5 Result(s)
within
Protocol
**ang-**g Kong
Cell Biology
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Protocol
Immunolabel-First-Expand-Later Expansion Microscopy Approach Using Stable STED Dyes
Multiple expansion microscopy approaches have been successfully used in the analysis of centrioles, centrosomes, and cilia, hel** to reveal the localization of numerous centrosomal and ciliary proteins at na...
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Protocol
Analyzing Centrioles and Cilia by Expansion Microscopy
Expansion microscopy is an imaging method based on isotropic physical expansion of biological samples, which improves optical resolution and allows imaging of subresolutional cellular components by conventiona...
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Protocol
Genetic Mouse Models: The Powerful Tools to Study Fat Tissues
Obesity and Type 2 diabetes (T2D) are associated with a variety of comorbidities that contribute to mortality around the world. Although significant effort has been expended in understanding mechanisms that mi...
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Protocol
Preparation of a Two-Photon Fluorescent Probe for Imaging H2O2 in Lysosomes in Living Cells and Tissues
Hydrogen peroxide (H2O2) plays important roles in many physiological and pathological processes. At the cellular organelle level, the abnormal concentrations of H2O2 in the lysosomes may cause redox imbalance and...
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Protocol
ENU-Based Phenotype-Driven Screening
Deciphering the contribution of individual genes and in turn pathways to cellular processes can be complicated and is often based on prior knowledge or assumptions of gene function. Phenotype-driven mutagenes...
