13 Result(s)
within
Protocol
**an Qi
Human Genetics
-
Protocol
Designing Overlap Extension PCR Primers for Protein Mutagenesis: A Programmatic Approach
Overlap extension PCR is one of the routinely used methods to generate mutagenic genes for the functional and structural study of proteins. However, it is time-consuming to design the overlap** mutagenic pri...
-
Protocol
Novel Methods for Family-Based Genetic Studies
The recent development of microarray and sequencing technology allows identification of disease susceptibility genes. Although the genome-wide association studies (GWAS) have successfully identified many genet...
-
Protocol
In Vivo Evaluation of Dystrophin Exon Skip** in mdx Mice
Dystrophin exon skip** in mdx mice has been the key model for the development of antisense therapy in vivo. Evaluation of exon skip** in this model involves the following two aspects: (1) efficiency and accur...
-
Protocol
Tagmentation-Based Library Preparation for Low DNA Input Whole Genome Bisulfite Sequencing
Aberrations of the DNA methylome contribute to onset and progression of diseases. Whole genome bisulfite sequencing (WGBS) is the only analytical method covering the complete methylome. Alternative methods req...
-
Protocol
Immunohistochemical Procedures for Characterizing the Retinal Expression Patterns of Cre Driver Mouse Lines
The retina is a thin neural tissue sitting on the backside of the eye, composed of light-sensing cells, interneurons, and output ganglion neurons. The latter send electrical signals to higher visual centers in...
-
Protocol
Competitive Immunoassays Using Antigen Microarrays
In this work, a non-fouling antigen competitive immunoassay microarray based on the polymer brush is reported to detect multiple mycotoxins. The detection is achieved by utilizing highly specific monoclonal an...
-
Protocol
Using Polymerase Preference Index to Design imLATE-PCR Primers for an Efficient Pyrosequencing
To simplify pyrosequencing protocol, we improved linear-after-the-exponential-PCR (imLATE-PCR) for yielding single-stranded DNA templates directly for pyrosequencing reaction. However, it is very critical for ...
-
Protocol
Pyrosequencing On-Chip Based on a Gel-Based Solid-Phase Amplification
As conventional solid-phase amplification (SPA) on a two-dimensional slide has a low amplification capacity due to a limited amount of immobilized primers, we propose a three-dimensional SPA by immobilizing pr...
-
Protocol
One Long Oligonucleotide or Two Short Oligonucleotides Based shRNA Construction and Expression
RNA interference (RNAi) has become a powerful and commonly used genetic tool for studying gene function. Vector-based short hairpin RNAs (shRNAs) trigger long-lasting RNAi effects compared to chemically synthe...
-
Protocol
Systemic Delivery of Antisense Oligomer in Animal Models and Its Implications for Treating DMD
Antisense oligomer (AO)-mediated splicing modulation for treating DMD demands a systemic administration of AOs as pharmacological drugs to achieve effective prevention of disease progression and to improve qua...
-
Protocol
MicroRNA Profiling Using µParaflo Microfluidic Array Technology
The diverse functions of microRNA (miRNA) molecules have drawn broad and intensive interest in various biological fields, biomedical applications, and technology development. Which are endogeneous cellular sho...
-
Protocol
Bacterial Delivery of siRNAs: A New Approach to Solid Tumor Therapy
RNAi is a powerful research tool for specific gene silencing and may also lead to promising novel therapeutic strategies. However, the development of RNAi-based therapies has been slow due to the lack of targe...
-
Protocol
μParaflo™ Biochip for Nucleic Acid and Protein Analysis
We describe in this chapter the use of oligonucleotide or peptide microarrays (arrays) based on microfluidic chips. Specifically, three major applications are presented: (1) microRNA/small RNA detection using ...
