12 Result(s)
within
Biotechnology
**an Qi
Protocol
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Protocol
In Vivo DNA Assembly Using the PEDA Method
Simple and efficient DNA assembly methods have been widely used in synthetic biology. Here, we provide the protocol for the recently developed PEDA (phage enzyme-assisted in vivo DNA assembly) method for direc...
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Protocol
A Directed Evolution Protocol for Engineering Minimal Transcription Factors, Based on CIS Display
Directed evolution is an efficient strategy for obtaining desired biomolecules. Since the 1990s, the emergence of display techniques has enabled high-throughput screening of functional proteins. However, class...
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Protocol
Quantifying ATP-Independent Nucleosome Chaperone Activity with Single-Molecule Methods
The dynamics of histone-DNA interactions govern chromosome organization and regulates the processes of transcription, replication, and repair. Accurate measurements of the energies and the kinetics of DNA bind...
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Designing Overlap Extension PCR Primers for Protein Mutagenesis: A Programmatic Approach
Overlap extension PCR is one of the routinely used methods to generate mutagenic genes for the functional and structural study of proteins. However, it is time-consuming to design the overlap** mutagenic pri...
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Protocol
Ligand-Directed GPCR Antibody Discovery
Develo** affinity reagents recognizing and modulating G-protein coupled receptors (GPCR) function by traditional animal immunization or in vitro screening methods is challenging. Some anti-GPCR antibodies ex...
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Structural Characterization of Silk Fibers by Small-Angle X-Ray Scattering
Nature is rich in all kinds of unbelievably designed microstructures, which endows the natural materials with various fantastic performances. Unveiling the mystery of the sophisticated configurations and the r...
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Discovery of Covalent Drugs Targeting the Key Enzymes of SARS-CoV-2 Using SCARdock
Historically, covalent drugs were avoided in drug development process due to the possible toxicity linked to the covalent binding of such drugs and cellular proteins. However, recent years have witnessed the f...
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Protocol
A Hybrid Biological–Biomaterial Vector for Antigen Delivery
A hybrid biological–biomaterial vector composed of a biocompatible polymeric biomaterial coating and an Escherichia coli core was designed and developed for antigen delivery. It provides a unique and efficient me...
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Protocol
Structural Characterization of Silk Fibers by Wide-Angle X-Ray Scattering
As one of the most advanced techniques to gain insight into the structure of the materials, wide-angle X-ray scattering (WAXS) records the scattering information at wide angles which typically larger than 5° (2θ)...
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Protocol
Engineering Human Neural Tissue by 3D Bioprinting
Bioprinting provides an opportunity to produce three-dimensional (3D) tissues for biomedical research and translational drug discovery, toxicology, and tissue replacement. Here we describe a method for fabrica...
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Protocol
Imaging Single Synaptic Vesicles in Mammalian Central Synapses with Quantum Dots
This protocol describes a sensitive and rigorous method to monitor the movement and turnover of single synaptic vesicles in live presynaptic terminals of mammalian central nerve system. This technique makes us...
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Protocol
μParaflo™ Biochip for Nucleic Acid and Protein Analysis
We describe in this chapter the use of oligonucleotide or peptide microarrays (arrays) based on microfluidic chips. Specifically, three major applications are presented: (1) microRNA/small RNA detection using ...
