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Protocol
Methods to Study Antagonistic Activities Among Oral Bacteria
Most bacteria in nature exist in multispecies communities known as biofilms. In the natural habitat where resources (nutrient, space, etc.) are usually limited, individual species must compete or collaborate w...
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Protocol
NTCP-Reconstituted In Vitro HBV Infection System
Sodium taurocholate cotransporting polypeptide (NTCP) has been identified as a functional receptor for hepatitis B virus (HBV). Expressing human NTCP in human hepatoma HepG2 cells (HepG2-NTCP) renders these ce...
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Protocol
Molecular Properties of Poliovirus Isolates: Nucleotide Sequence Analysis, Ty** by PCR and Real-Time RT-PCR
Virologic surveillance is essential to the success of the World Health Organization initiative to eradicate poliomyelitis. Molecular methods have been used to detect polioviruses in tissue culture isolates der...
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Protocol
Intraocular Delivery of Recombinant Virus
In the past 5 yr, advances in technology have been made that allow efficient somatic transfer of functional genes to target cells in the eye in vivo. The ability to deliver functional genes to these cells is d...
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Protocol
Transfection Assays for Transformation of Model Colonic Cell Lines
The recognition of cancer as a genetic disease has changed the approach investigators take to understanding the mechanisms of carcinogenesis. The discovery of oncogenes, and the recognition of the inactivation...