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    Protocol

    A Surgical Approach to Hindlimb Suspension: A Mouse Model of Disuse-Induced Atrophy

    Hindlimb suspension is a well-established rodent model of disuse-induced atrophy and is commonly used to simulate the effects of bed rest and space flight on humans. Over the decades, this method has undergone...

    Laura Kutz, Tong Zhou, Qi Chen, Hua Zhu in Chemokine-Glycosaminoglycan Interactions (2023)

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    Protocol

    A Fluorescence-Microscopic System for Monitoring the Turnover of the Autophagic Substrate p62/SQSTM1

    In conditions of cellular stress and nutrient shortage, macroautophagy (hereafter referred to as autophagy) assures the degradation of dysfunctional macromolecules and organelles as it liberates energy resourc...

    Hongzhong **, Qi Wu, Guido Kroemer, Oliver Kepp in Apoptosis and Cancer (2022)

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    Protocol

    Tetramer-Associated T Cell Receptor Sequencing

    Linking antigen specificity to T cell receptor (TCR) sequences is critical, albeit challenging, to both understanding T cell biology and develo** T cell-based therapeutics. Here, we describe in detail tetram...

    Michael Malone, Ke-Yue Ma, Shu-Qi Zhang, Ning Jiang in T-Cell Repertoire Characterization (2022)