71 Result(s)
within
Chapter
Lu**g Li
Biotechnology
Industrial Chemistry/Chemical Engineering
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Chapter
Cell-Free Display Techniques for Protein Evolution
Cell-free protein synthesis (CFPS) with flexibility and controllability can provide a powerful platform for high-throughput screening of biomolecules, especially in the evolution of peptides or proteins. In th...
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Biomanufacturing by In Vitro Biotransformation (ivBT) Using Purified Cascade Multi-enzymes
In vitro biotransformation (ivBT) refers to the use of an artificial biological reaction system that employs purified enzymes for the one-pot conversion of low-cost materials into biocommodities such as ethano...
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Producing N-Containing Chemicals from Biomass for High Performance Thermosets
Chemicals containing nitrogen atoms or N-containing chemicals (NCCs) are pivotal building blocks for polymers and composites that have high interest in technological fields, especially when they can be endowed...
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Downstream Processing of Medicinal Mushroom Products
Medicinal mushrooms are higher fungi that consist of ascomycetes, basidiomycetes, and imperfect fungi. They have been long used as tonic and traditional medicine in East Asia, Europe, and Africa. Contemporary ...
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Catalytic Upgrading of Bio-Based Ketonic Acids to Pyrrolidones with Hydrogen Donor Sources
Comprehensive utilization of biomass resources is necessary for the sustainable production of value-added chemicals and biofuels. For hydrogenative conversion processes, the type of hydrogen donor source among...
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Bottom-Up Synthetic Biology Using Cell-Free Protein Synthesis
Technical advances in biotechnology have greatly accelerated the development of bottom-up synthetic biology. Unlike top-down approaches, bottom-up synthetic biology focuses on the construction of a minimal cel...
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Recovery of Biohydrogen and Biomethane by Anaerobic Fermentation of Organic Solid Waste
Organic solid waste, which mainly consists of biowaste, around the world is increasing at an alarming rate. Much of the carbon in this biowaste originates from photosynthesis of atmospheric CO2. Long before human...
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Synthesis of Polyols and Organic Acids by Wild-Type and Metabolically Engineered Yarrowia lipolytica Strains
In the yeast Yarrowia lipolytica, sugar polyols and organic acids are derived from central metabolism, namely the citrate cycle and the pentose phosphate pathway. Although these metabolites have numerous applicat...
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Enhanced Biological Fixation of CO2 Using Microorganisms
Microbial fixation of carbon dioxide (CO2), represented by photosynthesis, is an important link of the global carbon cycle. It provides the majority of organic chemicals and energy for human consumption. With the...
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Integration of Waste Valorization for Sustainable Production of Chemicals and Materials via Algal Cultivation
Managing waste is an increasing problem globally. Microalgae have the potential to help remove contaminants from a range of waste streams and convert them into useful biomass. This article presents a critical ...
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Electrochemical Energy Production Using Fuel Cell Technologies
Fuel cells are highly efficient and environmentally friendly energy conversion devices that are receiving increasing attention and are steadily moving toward commercialization. Fuel cells deliver electricity a...
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Microalgae as a Source of Lutein: Chemistry, Biosynthesis, and Carotenogenesis
Microalgae represent a sustainable source of natural products, and over 15,000 novel compounds originated from algal biomass have been identified. This chapter focuses on algae-derived lutein, a group of high-...
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Current and Emerging Options for Taxol Production
Paclitaxel (trademark “Taxol”) is a plant-derived isoprenoid natural product that exhibits potent anticancer activity. Taxol was originally isolated from the Pacific yew tree in 1967 and triggered an intense s...
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Chapter and Conference Paper
The Application of Salting-Out in the Analysis of Methoxy-Phenolic Compounds in Pu-erh Tea
This paper presents the application of salting-out in the analysis of methoxy-phenolic compounds in pu-erh tea by using headspace solid microextraction (HS-SPME) combined with gas chromatography mass spectrome...
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Chapter and Conference Paper
The Effect of the Enzyme on the Liquid-State Fermentation of Pu’er Tea
In order to investigate the effect of the enzyme on the liquid-fermentation of sun dried green tea of Yunnan large leaf, three enzymes were used, including cellulase, pectinase, and flavourzyme. On the base of...
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Chapter and Conference Paper
Purification of Alkaline Pectinase in Engineering Bacillus subtilis
The alkaline pectinase in fermentation liquid of engineering Bacillus subtilis was isolated and purified by using centrifugation, ultrafiltration, ammonium sulfate precipitation, and ion exchange chromatograph...
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Chapter and Conference Paper
Improving the Production of Epothilones by Precursors Addition Based on Metabolic Pathway Analysis
Epothilones are a kind of poliketide macrolide with antifungal and anticancer bioactivity which are attracting more and more attention. However, despite a growing interest of epothilones, their practical use a...
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Chapter and Conference Paper
Cloning of Glucoamylase Gene from Aspergillus niger and its Expression in Saccharomyces cerevisiae W303-1B
To further research the heterologous expression of glucoamylase gene, Saccharomyces cerevisiae W303-1B as host strain to express the glucoamylase gene from Aspergillus niger was studied. Glucoamylase gene GAL was...
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Chapter and Conference Paper
Optimization Research of Sufu by Enzymatic Accelerated Maturation
Sufu, one of the famous traditional fermented soybean curds, was fast manufactured by maturing acceleration using enzymes. The optimum condition of enzymatic hydrolysis sufu was determined by the single factor...
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Chapter and Conference Paper
Synergistic Effects of Sakacin C2 in Combination with Food Preservatives
Synergistic effects of sakacin C2 in combination with nisin and several chemical preservatives against Staphylococcus aureus ATCC 63589 and Escherichia coli ATCC 25922 were investigated. Methyl paraben, propyl pa...
