13 Result(s)
within
Chapter
Klaas J. Hellingwerf
English
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Chapter
Challenges in the Application of Synthetic Biology Toward Synthesis of Commodity Products by Cyanobacteria via “Direct Conversion”
Cyanobacterial direct conversion of CO2 to several commodity chemicals has been recognized as a potential contributor to support the much-needed sustainable development of human societies. However, the feasibilit...
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Chapter
Photoreceptor Proteins from Purple Bacteria
Purple bacteria contain representatives of four of the six main families of photoreceptor proteins: phytochromes, BLUF domain containing proteins, xanthopsins (i.e., photoactive yellow proteins), and phototrop...
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Chapter
Light-induced behavioral responses (‘phototaxis’) in prokaryotes
Light-induced sensory responses are among the oldest scientific observations on bacterial behavior. Various types of response have been characterized physiologically in detail. However, the molecular basis of ...
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Chapter
Sensing of Green Light in Complementary Chromatic Adaptation of the Cyanobacterium Calothrix sp.
Light harvesting is essential for phototrophic organisms. The reaction centers of PS2 and PS1 in cyanobacteria contain chl a, † a ubiquitous pigment that absorbs light in two spectral domains. In the blue, absorp...
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Chapter
Global bioenergetics
The surface of the earth is subject to a continuous input of free enthalpy originating in the sun. A small, but important part of this free enthalpy is diverted, transiently, to physical chemical structures th...
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Chapter
Comparison of Retinal-Based and Chlorophyll-Based Photosynthesis: A Biothermokinetic Description of Photochemical Reaction Centers
Life on earth depends on the transformation of sunlight (electromagnetic radiation) into biologically useful chemical energy. The first step in this process is the conversion of the free energy from sunlight i...
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Chapter
Carotenoid Absorbance Changes in Liposomes Reconstituted with Pigment-Protein Complexes from Rhodobacter Sphaeroides
The electrochromic behaviour of carotenoids has been widely used to determine the electrical potential difference (∆ψ) across photosynthetic membranes, like chromatophores [1] and bacterial cells [2,3]. The ca...
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Chapter and Conference Paper
Functional Reconstitution of Photosynthetic Reaction Centre Complexes from Rhodopseudomonas Palustris
Reconstitution is a powerful technique in the elucidation of the mechanisms involved in membrane associated biological energy transduction (see for example Racker & Stoeckenius, 1974). For proton motive force ...
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Chapter
Light Driven Amino Acid Uptake in Membrane Vesicles of Streptococcus Cremoris Fused with Liposomes Containing Bacterial Reaction Centers
The incorporation of primary proton pumps in biological membranes has opened attractive possibilities for studies of proton motive force (Δp)-dependent processes in isolated membrane vesicles from bacterial1, and...
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Chapter
Light Induced Generation of a Proton Motive Force and Ca++-Transport in Membrane Vesicles of Streptococcus Cremoris Fused with Bacteriorhodopsin Proteoliposomes
The chemiosmotic hypothesis (Mitchell, 1966,1972) postulates that proton translocation by primary proton pumps generates an electrochemical proton gradient (∆p) across the cytoplasmic membrane. The electrochem...
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Chapter
The Electrochemical Proton Gradient and Solute Transport in Rhodopseudo-Monas Sphaeroides
Rhodopseudomonas sphaeroides is a Gram-negative phototrophic bacterium which can grow either aerobically in the dark or anaerobically in the light. The bacteria are surrounded by a cell-envelope ...
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Chapter
Reaction Centers from Rhodopseudomonas Sphaeroides in Reconstituted Phospholipid Vesicles. Structural Properties and Light-Dependent Proton Translocation
Reaction Centers (RC’s) from phototrophic bacteria catalyze light-driven transmembrane electron transfer as a first step in the (cyclic) electron transfer chain of such bacteria (for a review see: Okamura et a...
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Chapter
The Lactose Carrier of Escherichia Coli Functionally Incorporated in Rhodopseudomonas Sphaeroides Obeys the Regulatory Conditions of the Phototrophic Bacterium
Regulation of solute transport in Rps. sphaeroides is such that the presence of a proton motive force by itself is not enough for solute transport to take place. In addition linear or cyclic electron transfer is ...
