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Open AccessMolecular basis of Mg2+ permeation through the human mitochondrial Mrs2 channel
Mitochondrial RNA splicing 2 (Mrs2), a eukaryotic CorA ortholog, enables Mg2+ to permeate the inner mitochondrial membrane and plays an important role in mitochondrial metabolic function. However, the mechanism b...
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Article
Open AccessCalmodulin dissociates the STIM1-Orai1 complex and STIM1 oligomers
Store-operated calcium entry (SOCE) is a major pathway for calcium ions influx into cells and has a critical role in various cell functions. Here we demonstrate that calcium-bound calmodulin (Ca2+-CaM) binds to t...
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Article
Open AccessSynaptonemal complex protein 2 (SYCP2) mediates the association of the centromere with the synaptonemal complex
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Open AccessLeucine zipper-EF-hand containing transmembrane protein 1 (LETM1) forms a Ca2+/H+ antiporter
Leucine zipper-EF-hand-containing transmembrane protein1 (LETM1) is located in the mitochondrial inner membrane and is defective in Wolf-Hirschhorn syndrome. LETM1 contains only one transmembrane helix, but it...
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Open AccessCaM/BAG5/Hsc70 signaling complex dynamically regulates leaf senescence
Calcium signaling plays an essential role in plant cell physiology, and chaperone-mediated protein folding directly regulates plant programmed cell death. The Arabidopsis thaliana protein AtBAG5 (Bcl-2-associated...
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Open AccessThe structure of WbnH in a near active state
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Open AccessInside-out Ca2+ signalling prompted by STIM1 conformational switch
Store-operated Ca2+ entry mediated by STIM1 and ORAI1 constitutes one of the major Ca2+ entry routes in mammalian cells. The molecular choreography of STIM1–ORAI1 coupling is initiated by endoplasmic reticulum (E...
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Open AccessAngiomotin binding-induced activation of Merlin/NF2 in the Hippo pathway
The tumor suppressor Merlin/NF2 functions upstream of the core Hippo pathway kinases Lats1/2 and Mst1/2, as well as the nuclear E3 ubiquitin ligase CRL4DCAF1. Numerous mutations of Merlin have been identified in ...
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Article
Open AccessAuto-inhibitory Mechanism of the Human Mitochondrial RNase P Protein Complex
It is known that tRNAs play an essential role in genetic information transfer from DNA to protein. The maturation of tRNA precursors is performed by the endoribonuclease RNase P, which classically consists of ...
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Molecular mechanism of mitochondrial calcium uptake
Mitochondrial calcium uptake plays a critical role in various cellular functions. After half a century of extensive studies, the molecular components and important regulators of the mitochondrial calcium uptak...
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Article
Open AccessStructural Insight into the Central Element Assembly of the Synaptonemal Complex
The key step in meiosis is synaptonemal complex formation, which mediates homologous chromosome alignment and synapsis. False pairing between homologous chromosomes produces infertility. Here, we present a cry...
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Open AccessStructure of the kinase domain of human RNA-dependent protein kinase with K296R mutation reveals a face-to-face dimer
RNA-dependent protein kinase (PKR) is crucial for the innate immune response, cell growth, proliferation, signal transduction and apoptosis. The activation process of PKR has been studied for many years and is...
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Inhibition of the adenylyl cyclase toxin, edema factor, from Bacillus anthracis by a series of 18 mono- and bis-(M)ANT-substituted nucleoside 5′-triphosphates
Bacillus anthracis causes anthrax disease and exerts its deleterious effects by the release of three exotoxins, i.e. lethal factor, protective antigen and edema factor (EF), a highly acti...
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Structural insight into substrate specificity of human intestinal maltase-glucoamylase
Human maltase-glucoamylase (MGAM) hydrolyzes linear alpha-1,4-linked oligosaccharide substrates, playing a crucial role in the production of glucose in the human lumen and acting as an efficient drug target fo...
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Crystal structures of catalytic core domain of BIV integrase: implications for the interaction between integrase and target DNA
Integrase plays a critical role in the recombination of viral DNA into the host genome. Therefore, over the past decade, it has been a hot target of drug design in the fight against type 1 human immunodeficien...
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Crystal structures of catalytic and regulatory subunits of rat protein kinase CK2
Protein kinase CK2 consists of two catalytic subunits (CK2α) and two regulatory subunits (CK2β). Here, we report the crystal structures of rat CK2α mutant (rCK2α-ΔC, 1–335) and CK2β (rCK2β). The overall topolo...
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Structures of human insulin-degrading enzyme reveal a new substrate recognition mechanism
Insulin-degrading enzyme (IDE) is involved in the clearance of insulin and amyloid β. This is necessary to maintain proper blood sugar levels and to prevent accumulation of amyloid β in the brain. The crystal ...
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Crystallographic studies on the binding of coenzyme analogs to D-glyceraldehyde-3-phosphate dehydrogenase fromPalinurus versicolor
In contrast with the coezyme, two coenzyme analogs, ADP-ribose and SNAD, bind non-cooperatively to D-glyceraldehyde-3-phosphate dehydrogenase (GAPDH).Palinurus versicolor (PV) GAPDH complexed with ADP-ribose and ...
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Structure of D-glyceraldehyde-3-phosphate dehydrogenase fromPalinurus versicolor in a tetragonal crystal form
D-glyceraldehyde-3-phosphate dehydrogenase (holo-GAPDH) fromPalinurus versicolor was crystallized in a novel crystal form by the method of sitting-drop vapor diffusion. The crystals have space groupP4212, cell pa...