24 Result(s)
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Article
Open AccessEnhanced extracellular production of laccase in Coprinopsis cinerea by silencing chitinase gene
Laccase, a copper-containing polyphenol oxidase, is an important green biocatalyst. In this study, Laccase Lcc5 was homologous recombinantly expressed in Coprinopsis cinerea and a novel strategy of silencing chit...
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Article
Open AccessCRISPR–Cas9 applications in T cells and adoptive T cell therapies
T cell immunity is central to contemporary cancer and autoimmune therapies, encompassing immune checkpoint blockade and adoptive T cell therapies. Their diverse characteristics can be reprogrammed by different...
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Article
Open AccessStrand-selective base editing of human mitochondrial DNA using mitoBEs
A number of mitochondrial diseases in humans are caused by point mutations that could be corrected by base editors, but delivery of CRISPR guide RNAs into the mitochondria is difficult. In this study, we prese...
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Open AccessPSMC3 promotes RNAi by maintaining AGO2 stability through USP14
Argonaute 2 (AGO2), the only protein with catalytic activity in the human Argonaute family, is considered as a key component of RNA interference (RNAi) pathway. Here we performed a yeast two-hybrid screen using t...
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Open AccessGenomic prediction of drought tolerance during seedling stage in maize using low-cost molecular markers
Drought tolerance in maize is a complex and polygenic trait, especially in the seedling stage. In plant breeding, complex genetic traits can be improved by genomic selection (GS), which has become a practical ...
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Open AccessIdentification and fine map** of a major QTL (qRtsc8-1) conferring resistance to maize tar spot complex and validation of production markers in breeding lines
A major QTL of qRtsc8-1 conferring TSC resistance was identified and fine mapped to a 721 kb region on chromosome 8 at 81 Mb, and production markers were v...
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Open AccessBeat the stress: breeding for climate resilience in maize for the tropical rainfed environments
Intensive public sector breeding efforts and public-private partnerships have led to the increase in genetic gains, and deployment of elite climate-resilient maize cultivars for the stress-prone environments ...
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Open AccessStraw mulching and nitrogen application altered ammonia oxidizers communities and improved soil quality in the alkaline purple soil of southwest China
Microbe-mediated ammonia oxidation is a key process in soil nitrogen cycle. However, the effect of maize straw mulching on the ammonia oxidizers in the alkaline purple soil remains largely unknown. A three-yea...
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Publisher Correction: Glycosylase base editors enable C-to-A and C-to-G base changes
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
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Open AccessMaximizing efficiency of genomic selection in CIMMYT’s tropical maize breeding program
Historical data from breeding programs can be efficiently used to improve genomic selection accuracy, especially when the training set is optimized to subset individuals most informative of the target testing ...
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Open AccessGenomic prediction across years in a maize doubled haploid breeding program to accelerate early-stage testcross testing
Genomic selection with a multiple-year training population dataset could accelerate early-stage testcross testing by skip** the first-stage yield testing, which significantly saves the time a...
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Open AccessIdentification of Thermotoga maritima MSB8 GH57 α-amylase AmyC as a glycogen-branching enzyme with high hydrolytic activity
AmyC, a glycoside hydrolase family 57 (GH57) enzyme of Thermotoga maritima MSB8, has previously been identified as an intracellular α-amylase playing a role in either maltodextrin utilization or storage polysacch...
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Article
Correction to: Development of an autotrophic fermentation technique for the production of fatty acids using an engineered Ralstonia eutropha cell factory
The article Development of an autotrophic fermentation technique for the production of fatty acids using an engineered
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Article
Open AccessPerilipin 2 and lipid droplets provide reciprocal stabilization
The lipid droplet (LD)-associated protein adipose differentiation-related protein (ADRP or PLIN2) is required for the formation and stability of the LD organelle, whereas its biological roles are still obscure...
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Article
Open AccessA wheat protein kinase gene TaSnRK2.9-5A associated with yield contributing traits
We developed breeder-friendly high-throughput and cost-effective KASP marker for marker-assisted selection for grain yield related traits in wheat.
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Open AccessBiocatalytic activity of Monascus mycelia depending on physiology and high sensitivity to product concentration
Cell suspension culture using mycelia as whole cell biocatalyst for production of orange Monascus pigments has been carried out successfully in a nonionic surfactant micelle aqueous solution. Thus, selection of m...
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Open AccessEvaluation of a laying-hen tracking algorithm based on a hybrid support vector machine
Behavior is an important indicator reflecting the welfare of animals. Manual analysis of video is the most commonly used method to study animal behavior. However, this approach is tedious and depends on a subj...
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Article
Open AccessMolecular characterization of CIMMYT maize inbred lines with genoty**-by-sequencing SNPs
Molecular characterization information on genetic diversity, population structure and genetic relationships provided by this research will help maize breeders to better understand how to utiliz...
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Article
Open AccessSkeletal intramyocellular lipid metabolism and insulin resistance
Lipids stored in skeletal muscle cells are known as intramyocellular lipid (IMCL). Disorders involving IMCL and its causative factor, circulatory free fatty acids (FFAs), induce a toxic state and ultimately r...
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Article
Open AccessGenome sequence of cultivated Upland cotton (Gossypium hirsutum TM-1) provides insights into genome evolution
Two draft sequences of Gossypium hirsutum, the most widely cultivated cotton species, provide insights into genome structure, genome rearrangement, gene evolution and cotton fiber biology.
