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    Article

    A practical approach for O-linked mannose removal: the use of recombinant lysosomal mannosidase

    The methylotrophic yeast Pichia pastoris is an attractive expression system due to its ability to secrete large amounts of recombinant protein, with the potential for glycosylation. Advances in glycoengineering o...

    Daniel Hopkins, Sujatha Gomathinayagam in Applied Microbiology and Biotechnology (2015)

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    In vitro enzymatic treatment to remove O-linked mannose from intact glycoproteins

    The methylotrophic yeast Pichia pastoris is an attractive expression system for heterologous protein production due to its ability to perform posttranslational modifications, such as glycosylation, and secrete la...

    Sujatha Gomathinayagam, Stephen R. Hamilton in Applied Microbiology and Biotechnology (2014)

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    Article

    Elimination of diaminopeptidase activity in Pichia pastoris for therapeutic protein production

    Yeast are important production platforms for the generation of recombinant proteins. Nonetheless, their use has been restricted in the production of therapeutic proteins due to differences in their glycosylati...

    Daniel Hopkins, Sujatha Gomathinayagam in Applied Microbiology and Biotechnology (2014)