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165 Result(s)
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Article
Plant regeneration from rice (Oryza sativa L.) embryogenic suspension cells cryopreserved by vitrification
Rice cells were precultured for 10 d in medium containing 60 g/L sucrose and subsequently for 1 d in medium supplemented with 0. 4 M sorbitol. After loading with 25%PVS2 at 22°C for 10 min and dehydration in 1...
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Article
Use of oligonucleotide fingerprinting and faecal DNA in identifying the distribution of the Chinese tiger (Panthera tigris amoyensis Hilzheimer)
Chinese tiger (Panthera tigris amoyensis Hilzheimer) was once distributed in the Zhejiang Province, China, but there have not been any tiger sightings in this province since 1985. Fortunately, within the boundari...
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Article
Static magnetic field with a strong magnetic field gradient (41.7 T/m) induces c-jun expression in HL-60 cells
We investigated the effects of 6- and 10-T static magnetic fields (SMFs) on the expression of protooncogenes using Western blot immunohybridization methods. We used a SMF exposure system, which can expose cell...
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Article
Plant Regeneration from Hypocotyl Protoplasts of Red Cabbage (Brassica Oleracea) by Using Nurse Cultures
A protocol for rapid and efficient plant regeneration from protoplasts of red cabbage was developed by a novel nurse culture method. When the protoplasts of red cabbage were cultured in modified MS medium cont...
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Article
An identity-based key-exchange protocol
An identity-based key-exchange protocol using a bilinear map is proposed and it is proved SK-secure (session key secure) in the AM (authenticated links adversarial model) provided the BDDH (bilinear Diffie-Hel...
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Article
Liquefaction and product identification of main chemical compositions of wood in phenol
To clarify liquefaction ratios and their construction variations of the main chemical compositions of wood in phenol using phosphoric acid as a catalyst, the chemical ingredients of wood such as holocellulose,...
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Article
Factors affecting the resinification of liquefied phenolated wood
Wood of Chinese fir and poplar were liquefied in phenol at 150°C and atmospheric pressure. The liquefied wood were reacted with formaldehyde to synthesize the liquefied wood-based resin. The factors affecting ...
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Isolated and characterization of a cDNA encoding ethylene-responsive element binding protein (EREBP)/AP2-type protein, RCBF2, in Oryza sativa L.
A transcription factor RCBF2 which interacts with C-repeat/DRE was isolated from Oryza sativa L. by a yeast one-hybrid method. Analysis of the deduced RCBF2 amino acid sequence revealed that RCBF2 contained a con...
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Article
Preparation of liquefied wood-based resins and their application in molding material
To investigate value in use of liquefied wood-based resin applications in molding material, Chinese fir (Cunninghamia lanceolata) and poplar (Populus tomentosa) wood meal were liquefied in phenol. The reactant wa...
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Article
Isolation, optimization, and functional analysis of the cDNA encoding transcription factor OsDREB1B in Oryza Sativa L.
A previous study had indicated that the transcription factors DREB/CBF (DRE-binding protein/C-repeat binding factor) play important roles in the expression of many stress inducible genes under cold-temperature...
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Article
Molecular and biochemical characterization of Ba-EGA, a cellulase secreted by Bacillus sp. AC-1 from Ampullaria crosseans
A novel gene (Ba-ega) of Bacillus sp. AC-1, encoding an endoglucanase (Ba-EGA), was cloned and expressed in Escherichia coli. Ba-ega, containing a 1,980-bp open reading frame (ORF), encoded a protein of 659 amino...
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Article
α1,3 Fucosyltransferase-VII modifies the susceptibility of apoptosis induced by ultraviolet and retinoic acid in human hepatocarcinoma cells
The role of α1,3fucosyltransferase-VII (α1,3 FucT-VII) in cell apoptosis was studied in human hepatocellular carcinoma H7721 cells. After the cells were transfected with α1,3 FucT-VII cDNA, the expression of a...
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Article
Salicylic acid activates phenylalanine ammonia-lyase in grape berry in response to high temperature stress
Our previous work has indicated that salicylic acid (SA) is involved in the development of pea plant thermotolerance and induces the gene expression of phenylalanine ammonia-lyase (PAL; EC 4.3.1.5), a key enzy...
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Article
Open AccessDetection of Francisella tularensis in ticks and identification of their genotypes using multiple-locus variable-number tandem repeat analysis
Tularemia was reported in China over 50 years ago, however, many epidemical characteristics remain unclear. In the present study, the prevalence of Francisella tularensis in ticks was investigated during an epide...
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Article
Eighteen novel polymorphic microsatellite loci developed from the Père David’s deer (Elaphurus davidianus)
Père David’s deer is a severely bottlenecked species but without showing inbreeding depression, making it essential to develop molecular markers to explore her genetic mechanism of population recovery. In this...
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Article
GmRFP1 encodes a previously unknown RING-type E3 ubiquitin ligase in Soybean (Glycine max)
RING-finger proteins with E3 ubiquitin ligase activity play important roles in the regulation of plant growth and development. In this study, a cDNA clone encoding a novel RING-finger protein, designated as GmRFP...
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Evidence for programmed cell death and activation of specific caspase-like enzymes in the tomato fruit heat stress response
The tomato (Lycopersicon esculentum) fruit is the best available model to study the stress response of fleshy fruit. Programmed cell death (PCD) plays an important role in stress responses in mammals and plants. ...
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Article
Development and characterization of 14 novel microsatellite markers from the golden pheasant (Chrysolophus pictus)
Fourteen polymorphic microsatellite loci were isolated from the golden pheasant (Chrysolophus pictus). The average allele number of these microsatellites was 11.14 per locus, ranging from two to seventeen in a gr...
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Article
RA induces the neural-like cells generated from epigenetic modified NIH/3T3 cells
Recently, differentiated somatic cells had been reprogrammed to pluripotential state in vitro, and various tissue cells had been elicited from those cells. Epigenetic modifications allow differentiated cells t...
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Article
The polymorphisms of UCP1 genes associated with fat metabolism, obesity and diabetes
Uncoupling protein 1 (UCP1), a 32-kDa protein located in the inner mitochondrial membrane, is abundant in brown adipose tissue, as a proton transporter in mitochondria inner membrane which uncouples oxidative ...