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Article
Autofluorescence of Visual Receptors
SINCE 1878 when Kühne reported the faint bluish fluorescence of the dark adapted retina and its brighter greenish supplementation when the visual pigments were bleached1, these observations have been little used ...
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Article
Membrane structure changes in rod outer segments associated with rhodopsin bleaching
Static and time resolved birefringence analysis used to quantitate a fast membrane structure change driven by the formation of metarhodopsin II in intact frog retina. This change can be accounted for by the di...
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Article
Super dense carotenoid spectra resolved in single cone oil droplets
CONE visual receptors in reptiles and birds contain brilliant yellow, orange or red oil droplets, 3–6 µm in diameter, through which light must pass before reaching the visual pigment. The possible role of thes...
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Article
ATP mediates rapid reversal of cyclic GMP phosphodiesterase activation in visual receptor membranes
Weak or strong lights will activate visual receptor rod disk membrane (RDM) cyclic GMP phosphodiesterase (PDE) In the presence of GTP cofactor1,2. A similarly activated GTPase can exhaust small amounts of initial...
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Article
Acid polysaccharide content of frog rod outer segments determined by metachromatic toluidine blue staining
Sulfonation of periodate-oxidized vicinal hydroxyl groups on a polysaccharide backbone allows binding of toluidine blue (aldehyde bisulfite-toluidine blue or ABT staining) with a concurrent metachromatic shift...