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Article
Open AccessLSM14B controls oocyte mRNA storage and stability to ensure female fertility
Controlled mRNA storage and stability is essential for oocyte meiosis and early embryonic development. However, how to regulate mRNA storage and stability in mammalian oogenesis remains elusive. Here we showed...
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Article
Open AccessQuantitative sequencing using BID-seq uncovers abundant pseudouridines in mammalian mRNA at base resolution
Functional characterization of pseudouridine (Ψ) in mammalian mRNA has been hampered by the lack of a quantitative method that maps Ψ in the whole transcriptome. We report bisulfite-induced deletion sequencing...
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Article
Non-invasive urinary metabolomic profiles discriminate biliary atresia from infantile hepatitis syndrome
Neonatal cholestatic disorders are a group of hepatobiliary diseases occurring in the first 3 months of life. The most common causes of neonatal cholestasis are infantile hepatitis syndrome (IHS) and biliary a...
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Article
A method for isolating and culturing vascular smooth muscle cells from porcine cerebral arteries
Cerebral blood vessels have unique properties when compared to most peripheral vascular beds. One such property is that large cerebral vessels are involved in the regulation of cerebral vascular resistance. St...