161 Result(s)
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Article
Supplement of nutrients for effective cultivation of hepatitis B surface antigen-producing recombinant yeast
In the hepatitis B surface antigen (HBsAg)-producing recombinant yeast culture medium, the supply of Bacto-yeast nitrogen base without amino acids was found to be inadequate due to the lack of the several kind...
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Article
Expression of hepatitis B virus polyherase gene in E. coli.
The polymerase gene of the hepatitis B virus was fused with the E. coli Maltose Binding Protein (MBP) and expressed as a fusion protein. Reverse transcriptase activity was detected in purified P protein with the ...
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Article
Biological species and morphological characteristics ofArmillaria mellea complex in Hokkaido:A. sinapina and two new species,A. jezoensis andA. singula
Three intersterility groups ofArmillaria mellea sensu lato were discovered by examining all pairwise combinations of monosporous isolates of basidiomes collected in Hokkaido. One of them, group IV, was identified...
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Extracellular alkaline proteases from alkalophilic Vibrio metschnikovii strain RH530
Alkaline proteases, named VapT and VapK, from Gram-negative alkalophilic Vibrio metschnikovii strain RH530 were purified and characterized. Both enzymes had optimum pH and temperature of 10. 5 and 60 °C, respecti...
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Article
Controlled lysis ofE. coli double-lysogen by UV-irradiation
Sequential protein production and cell disruption was studied usingE. coli double-lysogen, P90c/(λHL1,ϕ434). The induction of the first prophage λHL1 to produce β-galactosidase by the temperature increase was fol...
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Article
Transcriptional control of the Saccharomyces cerevisiae ADH1 gene by autonomously replicating sequence binding factor 1
Autonomously replicating sequence (ARS)-binding factor 1 (ABF1) is a multifunctional protein involved in transcriptional activation and repression, as well as DNA replication, in yeast. The ADH1 gene, encoding al...
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Article
Biodegradation of methyl t-butyl ether by pure bacterial cultures
Three pure bacterial cultures degrading methyl t-butyl ether (MTBE) were isolated from activated sludge and fruit of the Gingko tree. They have been classified as belonging to the genuses Methylobacterium, Rhodo...
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Article
Screening and characterization of eicosapentaenoic acid-producing marine bacteria
To obtain eicosapentaenoic acid-producing bacteria, some 3,500 strains of bacteria were isolated from the intestine of marine animals from sea around the Korean peninsula. Forty two eicosapentaenoic acid-produ...
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Article
Interconversion and disproportionation reaction among cyclodextrin homologues in an aqueous two-phase-forming solution
Interconversion reactions of cyclodextrin glycosyltransferase (CGTase) among cyclodextrin (CD) homologues were experimentally investigated using each CD as a substrate in an aqueous, two-phase-forming polymer ...
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Article
Cloning of a New Bacillus thuringiensis cry1I-Type CrystalProtein Gene
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Article
Enantioselective production of levofloxacin by immobilized porcine liver esterase
Porcine liver esterase, which cleaves ofloxacin butyl ester enantioselectively to levofloxacin, was successfully immobilized in calcium alginate and polyacrylamide gel. Immobilized esterase in 5% (w/v) calcium...
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Article
Isolation and characterization of a clay-dispersing polysaccharide produced by the phytopathogenic fungus, Colletotrichum gloeosporioides
The fungus, Colletotrichum gloeosporioides, which is pathogenic to peppers produced an extracellular polysaccharide in liquid culture which possessed clay-dispersing activity. The polysaccharide could bind cation...
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Article
Preparation of a purified, inactivated Japanese encephalitis (JE) virus vaccine in Vero cells
An attenuated Japanese encephalitis (JE) virus SA14-14-2 (PDK) was adapted to Vero cells, a continuous cell line that has been licensed for human vaccine production, by serial passages. The resulting virus was...
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Article
Design and screening of in vivo expressed antimicrobial peptide library
The genetic library of α-helical amphipathic peptides, 20 amino acid long, was designed and expressed under the T7 promoter in the E. coli JM109(DE3) and BL21 (DE3). Clones that inhibited the growth of the host c...
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Article
Production of Linolenic Acid by Mortierella isabellina Grown on Octadecanol
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Article
Design of acoustic cell settler for filtering and recycling microbial cells
An acoustic cell settler (ACS) using ultrasound at cells of 3 MHz was used to recycle Saccharomyces cerevisiae in a fermenter. The locations of both the inlet and outlet in the acoustic cell settler, which have a...
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Article
A solid-state bioreactor coupled with forced aeration and pressure oscillation
A novel design of a solid-state bioreactor, operated with periodic pressure oscillation coupled with forced aeration through the medium, gave efficient control of temperature. The evaluation of the bioreactor ...
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Article
Neurospora crassa FKS Protein Binds to the (1,3)β-Glucan Synthase Substrate, UDP-Glucose
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Article
Antigenicity of the region encoded by exon8 of the human serine protease, HtrA2/Omi, is associated with its protein solubility
HtrA2/Omi, a mitochondrial serine protease, is pivotal in regulating apoptotic cell death. To determine the location of antigenic determinants in HtrA2/Omi, we expressed a series of the N-terminally truncated Htr...
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Article
Bioconversion of compactin into pravastatin by Streptomyces sp.
Streptomyces sp. Y-110, isolated from soil, modified compactin to pravastatin, a therapeutic agent for hypercholesterolemia. In a batch culture, the highest production of pravastatin was 340 mg l−1 from 750 mg c...