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  1. No Access

    Article

    Supplement of nutrients for effective cultivation of hepatitis B surface antigen-producing recombinant yeast

    In the hepatitis B surface antigen (HBsAg)-producing recombinant yeast culture medium, the supply of Bacto-yeast nitrogen base without amino acids was found to be inadequate due to the lack of the several kind...

    Kyung Hwan Jung, Mahn Hoon Park, Hong Mo Moon, Joon Shick Rhee in Biotechnology Letters (1991)

  2. No Access

    Article

    Expression of hepatitis B virus polyherase gene in E. coli.

    The polymerase gene of the hepatitis B virus was fused with the E. coli Maltose Binding Protein (MBP) and expressed as a fusion protein. Reverse transcriptase activity was detected in purified P protein with the ...

    Han Joo Lee, Yong Tae Kwon, Hyune Mo Rho, Guhung Jung in Biotechnology Letters (1993)

  3. No Access

    Article

    Extracellular alkaline proteases from alkalophilic Vibrio metschnikovii strain RH530

    Alkaline proteases, named VapT and VapK, from Gram-negative alkalophilic Vibrio metschnikovii strain RH530 were purified and characterized. Both enzymes had optimum pH and temperature of 10. 5 and 60 °C, respecti...

    Yong Tae Kwon, ** Oh Kim, Sun Young Moon, Hyune Hwan Lee… in Biotechnology Letters (1994)

  4. No Access

    Article

    Controlled lysis ofE. coli double-lysogen by UV-irradiation

    Sequential protein production and cell disruption was studied usingE. coli double-lysogen, P90c/(λHL1,ϕ434). The induction of the first prophage λHL1 to produce β-galactosidase by the temperature increase was fol...

    Soon-Suk Park, Yoon-Mo Koo in Biotechnology Letters (1994)

  5. No Access

    Article

    Screening and characterization of eicosapentaenoic acid-producing marine bacteria

    To obtain eicosapentaenoic acid-producing bacteria, some 3,500 strains of bacteria were isolated from the intestine of marine animals from sea around the Korean peninsula. Forty two eicosapentaenoic acid-produ...

    Ki Woong Cho, Sang Jun Mo in Biotechnology Letters (1999)

  6. No Access

    Article

    Interconversion and disproportionation reaction among cyclodextrin homologues in an aqueous two-phase-forming solution

    Interconversion reactions of cyclodextrin glycosyltransferase (CGTase) among cyclodextrin (CD) homologues were experimentally investigated using each CD as a substrate in an aqueous, two-phase-forming polymer ...

    Woo-** Chang, Yoon-Mo Koo in Biotechnology Letters (2000)

  7. No Access

    Article

    Enantioselective production of levofloxacin by immobilized porcine liver esterase

    Porcine liver esterase, which cleaves ofloxacin butyl ester enantioselectively to levofloxacin, was successfully immobilized in calcium alginate and polyacrylamide gel. Immobilized esterase in 5% (w/v) calcium...

    Sang-Yoon Lee, Byung-Hyuk Min, Sung-Ho Hwang, Yoon-Mo Koo… in Biotechnology Letters (2001)

  8. No Access

    Article

    Isolation and characterization of a clay-dispersing polysaccharide produced by the phytopathogenic fungus, Colletotrichum gloeosporioides

    The fungus, Colletotrichum gloeosporioides, which is pathogenic to peppers produced an extracellular polysaccharide in liquid culture which possessed clay-dispersing activity. The polysaccharide could bind cation...

    Sang Ho Park, Chang Won Choi, Myoung Yong Shim, Won Mok Park… in Biotechnology Letters (2001)

  9. Article

    Preparation of a purified, inactivated Japanese encephalitis (JE) virus vaccine in Vero cells

    An attenuated Japanese encephalitis (JE) virus SA14-14-2 (PDK) was adapted to Vero cells, a continuous cell line that has been licensed for human vaccine production, by serial passages. The resulting virus was...

    Sun Pyo Hong, Wang-Don Yoo, Robert Putnak, Ashok K. Srivastava… in Biotechnology Letters (2001)

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  10. No Access

    Article

    Design and screening of in vivo expressed antimicrobial peptide library

    The genetic library of α-helical amphipathic peptides, 20 amino acid long, was designed and expressed under the T7 promoter in the E. coli JM109(DE3) and BL21 (DE3). Clones that inhibited the growth of the host c...

    Kyoung-Chul Choi, Hyeung-Rak Kim, Young-Soo Park, Sang-Mo Park… in Biotechnology Letters (2002)

  11. No Access

    Article

    Design of acoustic cell settler for filtering and recycling microbial cells

    An acoustic cell settler (ACS) using ultrasound at cells of 3 MHz was used to recycle Saccharomyces cerevisiae in a fermenter. The locations of both the inlet and outlet in the acoustic cell settler, which have a...

    Sung-Ho Hwang, Yoon-Mo Koo in Biotechnology Letters (2003)

  12. No Access

    Article

    A solid-state bioreactor coupled with forced aeration and pressure oscillation

    A novel design of a solid-state bioreactor, operated with periodic pressure oscillation coupled with forced aeration through the medium, gave efficient control of temperature. The evaluation of the bioreactor ...

    **aoyong Zhang, Haitao Mo, Jian'an Zhang, Zuohu Li in Biotechnology Letters (2003)

  13. No Access

    Article

    Antigenicity of the region encoded by exon8 of the human serine protease, HtrA2/Omi, is associated with its protein solubility

    HtrA2/Omi, a mitochondrial serine protease, is pivotal in regulating apoptotic cell death. To determine the location of antigenic determinants in HtrA2/Omi, we expressed a series of the N-terminally truncated Htr...

    Hyo-** Park, Ju-Youn Choi, Young-Mo Seong, Chul Han… in Biotechnology Letters (2003)

  14. No Access

    Article

    Bioconversion of compactin into pravastatin by Streptomyces sp.

    Streptomyces sp. Y-110, isolated from soil, modified compactin to pravastatin, a therapeutic agent for hypercholesterolemia. In a batch culture, the highest production of pravastatin was 340 mg l−1 from 750 mg c...

    Joo-Woong Park, Joo-Kyung Lee, Tae-Jong Kwon, Dong-Hee Yi… in Biotechnology Letters (2003)

  15. No Access

    Article

    Thermostable Alkaline Protease from a Novel Marine Haloalkalophilic Bacillus Clausii Isolate

    An oxidative and SDS-stable alkaline protease secreted by a marine haloalkalophilic Bacillus clausii isolated from the tidal mud flats of the Korean Yellow Sea near Inchon City was investigated in batch fermentat...

    C. Ganesh Kumar, Han-Seung Joo, Yoon-Mo Koo… in World Journal of Microbiology and Biotechn… (2004)

  16. No Access

    Article

    Adverse effect of chloride impurities on lipase-catalyzed transesterifications in ionic liquids

    The adverse influence of chloride impurities on the lipase-catalyzed transesterification in ionic liquid is described. The activity of lipase from Rhizomucor miehei exponentially decreased with increasing Cl− con...

    Sang Hyun Lee, Sung Ho Ha, Sun Bok Lee, Yoon-Mo Koo in Biotechnology Letters (2006)

  17. No Access

    Article

    Pretreatment of lipase with soybean oil before immobilization to prevent loss of activity

    Lipase was pretreated with soybean oil in order to allow fatty acids to bond to the active site before immobilization. This pretreated lipase exhibited steric hindrance around the active site such that during ...

    Dong Hwan Lee, Jung Mo Kim, Seong Woo Kang, Ji Won Lee… in Biotechnology Letters (2006)

  18. No Access

    Article

    Analysis of functional properties of Lactobacillus acidophilus

    Metabolites from Lactobacillus acidophilus were analysed. The results showed that Lactobacillus acidophilus Ind-1 and Lactobacillus acidophilus Lakcid produced respectively 12.73 g and 13....

    Ruixiang Zhao, Junliang Sun, Haizhen Mo… in World Journal of Microbiology and Biotechn… (2007)

  19. No Access

    Article

    Zearalenone (ZEN) detection by a single chain fragment variable (scFv) antibody

    Zearalenone (ZEN) is a mycotoxin produced by members of the genus Fusarium. ZEN detection by ELISA using single chain fragment variable (scFv) represents a novel means of mycotoxin detection and diagnosis of f...

    Shi-Hua Wang, **ao-Yu Du, Ling Lin… in World Journal of Microbiology and Biotechn… (2008)

  20. No Access

    Article

    A novel extracellular phospholipase C purified from a marine bacterium, Pseudoalteromonas sp. J937

    Marine bacterial isolates were screened for phospholipase C (PLC) activity on PCY agar plates containing phosphatidylcholine (PC) as substrate. The strain that showed the highest activity on a PCY screening ag...

    SangJoon Mo, Jae-heon Kim, Ki Woong Cho in Biotechnology Letters (2009)

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