27 Result(s)
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Article
Jiahai Zhou
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Article
Accelerating the design of pili-enabled living materials using an integrative technological workflow
Bacteria can be programmed to create engineered living materials (ELMs) with self-healing and evolvable functionalities. However, further development of ELMs is greatly hampered by the lack of engineerable non...
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Tandem intermolecular [4 + 2] cycloadditions are catalysed by glycosylated enzymes for natural product biosynthesis
Tandem Diels–Alder reactions are frequently used in the construction of polycyclic ring systems in complex organic compounds. Unlike the many Diels−Alderases (DAases) that catalyse a single cycloaddition, enzy...
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Enzymatic catalysis favours eight-membered over five-membered ring closure in bicyclomycin biosynthesis
The construction of O-heterocycles is an important step in organic synthesis and biosynthesis for producing valuable ring compounds. Although enzyme-catalysed five- or six-membered ring closures in O-heterocyc...
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Photoinduced chemomimetic biocatalysis for enantioselective intermolecular radical conjugate addition
Exploiting nature’s catalysts for non-natural transformations that are inaccessible to chemocatalysis is highly desirable but challenging. On the one hand, the widespread nicotinamide-dependent oxidoreductases...
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Open AccessStructural and mechanistic basis of the high catalytic activity of monooxygenase Tet(X4) on tigecycline
Tigecycline is a tetracycline derivative that constitutes one of the last-resort antibiotics used clinically to treat infections caused by both multiple drug-resistant (MDR) Gram-negative and Gram-positive bac...
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Open AccessReductive inactivation of the hemiaminal pharmacophore for resistance against tetrahydroisoquinoline antibiotics
Antibiotic resistance is becoming one of the major crises, among which hydrolysis reaction is widely employed by bacteria to destroy the reactive pharmacophore. Correspondingly, antibiotic producer has canonic...
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An enzymatic Alder-ene reaction
An ongoing challenge in chemical research is to design catalysts that select the outcomes of the reactions of complex molecules. Chemists rely on organocatalysts or transition metal catalysts to control stereo...
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Open AccessCorrection to: Structure-guided engineering of a Thermobifida fusca cutinase for enhanced hydrolysis on natural polyester substrate
An amendment to this paper has been published and can be accessed via the original article.
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Open AccessStructure-guided engineering of a Thermobifida fusca cutinase for enhanced hydrolysis on natural polyester substrate
Cutinases could degrade insoluble polyester, including natural cutin and synthetic plastic. However, their turnover efficiency for polyester remains too low for industrial application. Herein, we report the 1....
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Structural basis for stereoselective dehydration and hydrogen-bonding catalysis by the SAM-dependent pericyclase LepI
LepI is an S-adenosylmethionine (SAM)-dependent pericyclase that catalyses the formation of the 2-pyridone natural product leporin C. Biochemical characterization has shown that LepI can catalyse stereoselective ...
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Open AccessArtificial cysteine-lipases with high activity and altered catalytic mechanism created by laboratory evolution
Engineering artificial enzymes with high activity and catalytic mechanism different from naturally occurring enzymes is a challenge in protein design. For example, many attempts have been made to obtain active...
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Structure-based design of agarase AgWH50C from Agarivorans gilvus WH0801 to enhance thermostability
AgWH50C, an exo-β-agarase of GH50 isolated from Agarivorans gilvus WH0801, plays a key role in the enzymatic production of neoagarobiose, which has great application prospect in the cosmetics and pharmaceutical i...
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Enhancement of ε-poly-l-lysine production by overexpressing the ammonium transporter gene in Streptomyces albulus PD-1
The antibacterial polymer ɛ-poly-l-lysine (ε-PL) has been widely used as a safe food preservative. As the synthesis of ε-PL requires a rich supply of nitrogen, the efficiency of nitrogen translocation and utiliza...
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Resistance-gene-directed discovery of a natural-product herbicide with a new mode of action
Bioactive natural products have evolved to inhibit specific cellular targets and have served as lead molecules for health and agricultural applications for the past century1–3. The post-genomics era has brought a...
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Open AccessGyrI-like proteins catalyze cyclopropanoid hydrolysis to confer cellular protection
GyrI-like proteins are widely distributed in prokaryotes and eukaryotes, and recognized as small-molecule binding proteins. Here, we identify a subfamily of these proteins as cyclopropanoid cyclopropyl hydrola...
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Structural basis of nonribosomal peptide macrocyclization in fungi
Unlike their bacterial counterparts, fungal nonribosomal peptide synthetases utilize a terminal condensation-like (CT) domain to form macrocycles, details of which are illuminated by structures of a CT domain and...
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l-Ribose isomerase and mannose-6-phosphate isomerase: properties and applications for l-ribose production
l-Ribose is a synthetic l-form monosaccharide. It is a building block of many novel nucleotide analog anti-viral drugs. Bio-production of l-ribose relies on a two-step reaction: (i) conve...
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Open AccessAn unusual UMP C-5 methylase in nucleoside antibiotic polyoxin biosynthesis
Polyoxin is a group of structurally-related peptidyl nucleoside antibiotics bearing C-5 modifications on the nucleoside skeleton. Although the structural diversity and bioactivity preference of polyoxin are, t...
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Open AccessCrystal structure of tyrosine decarboxylase and identification of key residues involved in conformational swing and substrate binding
Tyrosine decarboxylase (TDC) is a pyridoxal 5-phosphate (PLP)-dependent enzyme and is mainly responsible for the synthesis of tyramine, an important biogenic amine. In this study, the crystal structures of the...
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Open AccessMarked enhancement of Acinetobacter sp. organophosphorus hydrolase activity by a single residue substitution Ile211Ala
The activity of organophosphorus hydrolase (OPH) that catalyzes the hydrolysis of neurotoxic organophosphates (OPs) was reported to evolve from lactonase.
