585 Result(s)
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Article
Direct binding of G-protein βλ complex to voltage-dependent calcium channels
Voltage-dependent Ca2+ channels play a central role in controlling neurotransmitter release at the synapse1,2. They can be inhibited by certain G-protein-coupled receptors, acting by a pathway intrinsic to the me...
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Article
Crustal structure in western Tibetan Plateau
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Synthesis and crystal structure of SrNixV6-x O11
In SrV6O11 the M(3) sites are mainly occupied by trivalent cations; while M(l) and M (2) sites are taken by mixing of trivalent and tetravalent cations, the content of tetravalent is higher than that of trivalent...
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Study on uranium I odd parity energy levels using chemical pattern recognition techniques
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Article
Cloning of theAspergillus awamori glucoamylase gene and expression inSaccharomyces cerevisiae
TheA. awamori glucoamylase I gene was obtained by RT-PCR and inserted into the plasmid pAC1. We constructed an expression vector pAC1-CA, which was used to transform the yeast S.cerevisiae AS 2.1364 without auxot...
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Article
Secretory expression of the fusion protein composed of human IL-2 and PreS antigen of hepatitis B virus in mammalian cells
In order to make entire HBV pmSAg secrete from mammalian cells, we conatmcted an eukaryotic expression vector by using leader sequence of human interleukin-2 (IL-2) as secretory signal peptide, and using high ...
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Article
Production of human neurotrophin-3 in baculovirus expression system
Human NT-3 gene was inserted into pPAK9 and the resultant plasmid was used to co-transfect sf21 cells with Bsu36 I digested Bac6 genomic DNA. Recombinant baculovirus was isolated by plaque assay. Slot-hybridiz...
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Expression of human β-subunit nerve growth factor (hNGFB) in yeastPichia pastoris andE. coli
The gene hNGFB encoding the β subunit of human nerve growth factor (hNGF) was cloned intoP. pastoris secretive expression vector pHIL-S1 andE. coli expression vector pET-15b. The recombinant hNGFB vectors pSNGF a...
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Phase-shifting technique applied to circular harmonic-based joint transform correlator
The phase-shifting technique is applied to the circular harmonic expansion-based joint transform correlator. Computer simulation has shown that the light efficiency and the discrimination capability are greatl...
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Article
Inverse kinematics model of parallel macro-micro manipulator system
An improved design, which employs the integration of optic, mechanical and electronic technologies for the next generation large radio telescope, is presented in this note. The authors propose the concept of p...
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Article
A novel differential velocity modulation laser spectroscopy
Experimental investigation of a novel differential velocity modulation laser spectroscopy is reported and demonstrated with the spectra of n 2 + A2Dm -X2Ó ...
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Article
Research on crystal growth and properties of a new nonlinear optical crystal: Bismuth borate BiB3O6
BiB3O6 (BIBO) single crystals with sizes of 44 mm × 24 mm × 10 mm and weight of 24.7 g have been successfully grown by the top-seeded method. In this note, the preparation of seed, the growth of crystal and some ...
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Article
Decay dynamics of excited CS2 and NH3 investigated by femtosecond laser
By using the home-made femtosecond laser system and the time-of-flight mass spectrometer, the decay dynamics of excited carbon disulfide (CS2) and ammonia (NH3) are investigated in real time by pump-probe multiph...
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Article
Effects of calmodulin on DNA-binding activity of heat shock transcription factorin vitro
The DNA-binding activity of heat shock transcription factor (HSF) was induced by heat shock (HS) of a whole cell extract. Addition of antiserum, specific to CaM, to a whole cell extract reduced bind of the HSF...
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A complete sequence and comparative analysis of a SARS-associated virus (Isolate BJ01)
The genome sequence of the Severe Acute Respiratory Syndrome (SARS)-associated virus provides essential information for the identification of pathogen(s), exploration of etiology and evolution, interpretation ...
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Article
Comparative genome analysis of deleted genes in Shigella flexneri 2a strain 301
Comparative genome analysis is performed between Shigella flexneri 2a strain 301 and its close relatives, the non-pathogenic E. coli K-12 strain MG1655. Result shows that there are 136 DNA segments whose size is ...
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Article
Study of an athermal infrared dual band optical system design containing harmonic diffractive element
A harmonic diffractive element (HDE) is first successfully introduced to the athermal system of infrared dual band in this paper. In this system, there are only three lens and two materials, silicon and german...
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Contributions of different land cover types in Otindag Sandy Land and Bashang area of Hebei Province to the material source of sand stormy weather in Bei**g
Different land cover types in Otindag Sandy Land and Bashang area of Hebei Province are linked to a material source of sand stormy weather in Bei**g based on results of field vegetation and soil survey and la...
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Analysis of components of conserved “backbone sequences” among genomes ofShigella spp. strains
Difference in the genomic compositions of prokaryotes is the basis of the diversity in their biological characters. However, besides their flora- or strain-specific genes, those floras with closer relationship...
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Experimental investigation of integrated air purifying technology for bioaerosol removal and inactivation in central air-conditioning system
In this research, high voltage static electricity and ultraviolet technologies were integrated to an air purifying device which can be used to trap and kill airborne bacteria and viruses in central air-conditi...