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Production of human neurotrophin-3 in baculovirus expression system

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Chinese Science Bulletin

Abstract

Human NT-3 gene was inserted into pPAK9 and the resultant plasmid was used to co-transfect sf21 cells with Bsu36 I digested Bac6 genomic DNA. Recombinant baculovirus was isolated by plaque assay. Slot-hybridization, immunocytochemistry and serum-free culture of DRG demonstrated that NT-3 had been integrated into Bac6 DNA, expressed and secreted. The yield of purified hNT-3 was 200 μg from 200 mL cultural supernatant after passing CM Sepharose Fast Flow column, the concentration for its maximum bioactivity was 4 ng/mL.

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Authors and Affiliations

  1. Institute of Basic Medical Sciences, Academy of Military Medical Sciences, 100850, Bei**g, China

    Yuanyue Mu, Hong Liu, Jie Zhang, Shuhong Liu, Yan Wu, Jiazheng Wang & Ming Fan

Authors
  1. Yuanyue Mu
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  2. Hong Liu
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  3. Jie Zhang
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  4. Shuhong Liu
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  5. Yan Wu
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  6. Jiazheng Wang
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  7. Ming Fan
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Mu, Y., Liu, H., Zhang, J. et al. Production of human neurotrophin-3 in baculovirus expression system. Chin. Sci. Bull. 43, 1562–1566 (1998). https://doi.org/10.1007/BF02883449

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  • DOI: https://doi.org/10.1007/BF02883449

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