36 Result(s)
within
Microbial Genetics and Genomics
Guo-**n Jiang
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Open AccessIn silico and microarray-based genomic approaches to identifying potential vaccine candidates against Leptospira interrogans
Currently available vaccines against leptospirosis are of low efficacy, have an unacceptable side-effect profile, do not induce long-term protection, and provide no cross-protection against the different serov...
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Open AccessGenetic diversity among major endemic strains of Leptospira interrogans in China
Leptospirosis is a world-widely distributed zoonosis. Humans become infected via exposure to pathogenic Leptospira spp. from contaminated water or soil. The availability of genomic sequences of Leptospira interro...
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Screening of a high growth influenza B virus strain in Vero cells
Due to the insufficient supply of embryonated chicken eggs, the preparation of large quantities of inactivated influenza vaccines will require an alternative virus culture system after the emergence or reemerg...
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Open AccessIdentification and characterization of a novel fumarase gene by metagenome expression cloning from marine microorganisms
Fumarase catalyzes the reversible hydration of fumarate to L-malate and is a key enzyme in the tricarboxylic acid (TCA) cycle and in amino acid metabolism. Fumarase is also used for the industrial production of L
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Molecular characterization and comparative analysis of two waxy alleles in barley
Two alleles of the barley waxy locus were characterized from non-waxy cultivar Bowman and waxy cultivar CDC Candle, respectively. Their nucleotide and protein sequences were compared with other known waxy genes. ...
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Establishment, growth kinetics, and susceptibility to AcMNPV of heat tolerant lepidopteran cell lines
Lepidopteran heat-tolerant (ht) cell lines have been obtained with sf-9, sf-21 and several Bombyx cells. They have a distinct karyotype, membrane lipid composition, morphology and growth kinetics from the parenta...
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Improved expression of secretory and trimeric proteins in mammalian cells via the introduction of a new trimer motif and a mutant of the tPA signal sequence
Ideal immunogenicity in antigens is a prerequisite to eliciting a sufficiently strong immune and memory response via either DNA or protein vaccines. To improve immunogenicity, efforts have focused on high-leve...
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Susceptibility to AcMNPV and expression of recombinant proteins by a novel cell clone derived from a Trichoplusia ni QAU-BTI-Tn9-4s cell line
It is well known that Tn5B1-4 (commercially known as the High Five) cell line is highly susceptible to baculovirus and provides superior production of recombinant proteins when compared to other insect cell li...
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Genetic characteristics of 2009 pandemic H1N1 influenza a viruses isolated from Mainland China
A total of 100 H1N1 flu real-time-PCR positive throat swabs collected from fever patients in Zhejiang, Hubei and Guangdong between June and November 2009, were provided by local CDC laboratories. After MDCK ce...
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Complete mitochondrial genomes of two cockroaches, Blattella germanica and Periplaneta americana, and the phylogenetic position of termites
The mitochondrial genomes are one of the most information-rich markers in phylogenetics. The relationships within superorder Dictyoptera have been debated in the literature. However, the closely related termit...
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Open AccessHyperproduction of poly(4-hydroxybutyrate) from glucose by recombinant Escherichia coli
Poly(4-hydroxybutyrate) [poly(4HB)] is a strong thermoplastic biomaterial with remarkable mechanical properties, biocompatibility and biodegradability. However, it is generally synthesized when 4-hydroxybutyra...
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The full-length genome analysis of a street rabies virus strain isolated in Yunnan province of China
The epidemic of rabies has rapidly increased and expanded in Yunnan province in recent years. In order to further analyze and understand the etiological reasons for the rapid expansion of rabies in Yunnan, a s...
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Identification of differentially expressed genes in American cockroach ovaries and testes by suppression subtractive hybridization and the prediction of its miRNAs
Studies on the cockroach have contributed to our understanding of several important developmental processes, especially those that can be easily studied in the embryo. However, our knowledge on late events suc...
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Open AccessGlobal analysis of the differentially expressed miRNAs of prostate cancer in Chinese patients
Our recent study showed the global physiological function of the differentially expressed genes of prostate cancer in Chinese patients was different from that of other non-Chinese populations. microRNA are est...
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Open AccessMetabolic flux responses to genetic modification for shikimic acid production by Bacillus subtilis strains
Shikimic acid (SA) is a key chiral starting molecule for the synthesis of the neuramidase inhibitor GS4104 against viral influenza. Microbial production of SA has been extensively investigated in Escherichia coli
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Open AccessHigh throughput sequencing of two celery varieties small RNAs identifies microRNAs involved in temperature stress response
MicroRNAs (miRNAs) are small, non-coding RNAs of 20 to 24 nucleotides that regulate gene expression and responses to biotic and abiotic stress. Till now, no reports have previously been published concerning mi...
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Open AccessComplete genome sequence and comparative genomic analyses of the vancomycin-producing Amycolatopsis orientalis
Amycolatopsis orientalis is the type species of the genus and its industrial strain HCCB10007, derived from ATCC 43491, has been used for large-scale production of the vital antibiotic vancomycin. However, to dat...
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Open AccessGenome organisation of the Acinetobacter lytic phage ZZ1 and comparison with other T4-like Acinetobacter phages
Phage ZZ1, which efficiently infects pathogenic Acinetobacter baumannii strains, is the fifth completely sequenced T4-like Acinetobacter phage to date. To gain a better understanding of the genetic characteristic...
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Identification and validation of quantitative trait loci for seed yield, oil and protein contents in two recombinant inbred line populations of soybean
Soybean seeds contain high levels of oil and protein, and are the important sources of vegetable oil and plant protein for human consumption and livestock feed. Increased seed yield, oil and protein contents a...
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Open AccessGenome-wide association study for flowering time, maturity dates and plant height in early maturing soybean (Glycine max) germplasm
Soybean (Glycine max) is a photoperiod-sensitive and self-pollinated species. Days to flowering (DTF) and maturity (DTM), duration of flowering-to-maturity (DFTM) and plant height (PH) are crucial for soybean ada...
