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Open AccessIn silico and microarray-based genomic approaches to identifying potential vaccine candidates against Leptospira interrogans
Currently available vaccines against leptospirosis are of low efficacy, have an unacceptable side-effect profile, do not induce long-term protection, and provide no cross-protection against the different serov...
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Article
Open AccessGenetic diversity among major endemic strains of Leptospira interrogans in China
Leptospirosis is a world-widely distributed zoonosis. Humans become infected via exposure to pathogenic Leptospira spp. from contaminated water or soil. The availability of genomic sequences of Leptospira interro...
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Article
Screening of a high growth influenza B virus strain in Vero cells
Due to the insufficient supply of embryonated chicken eggs, the preparation of large quantities of inactivated influenza vaccines will require an alternative virus culture system after the emergence or reemerg...
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Article
Open AccessIdentification and characterization of a novel fumarase gene by metagenome expression cloning from marine microorganisms
Fumarase catalyzes the reversible hydration of fumarate to L-malate and is a key enzyme in the tricarboxylic acid (TCA) cycle and in amino acid metabolism. Fumarase is also used for the industrial production of L
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Improved expression of secretory and trimeric proteins in mammalian cells via the introduction of a new trimer motif and a mutant of the tPA signal sequence
Ideal immunogenicity in antigens is a prerequisite to eliciting a sufficiently strong immune and memory response via either DNA or protein vaccines. To improve immunogenicity, efforts have focused on high-leve...
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Genetic characteristics of 2009 pandemic H1N1 influenza a viruses isolated from Mainland China
A total of 100 H1N1 flu real-time-PCR positive throat swabs collected from fever patients in Zhejiang, Hubei and Guangdong between June and November 2009, were provided by local CDC laboratories. After MDCK ce...
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Open AccessHyperproduction of poly(4-hydroxybutyrate) from glucose by recombinant Escherichia coli
Poly(4-hydroxybutyrate) [poly(4HB)] is a strong thermoplastic biomaterial with remarkable mechanical properties, biocompatibility and biodegradability. However, it is generally synthesized when 4-hydroxybutyra...
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Article
Open AccessGlobal analysis of the differentially expressed miRNAs of prostate cancer in Chinese patients
Our recent study showed the global physiological function of the differentially expressed genes of prostate cancer in Chinese patients was different from that of other non-Chinese populations. microRNA are est...
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Open AccessMetabolic flux responses to genetic modification for shikimic acid production by Bacillus subtilis strains
Shikimic acid (SA) is a key chiral starting molecule for the synthesis of the neuramidase inhibitor GS4104 against viral influenza. Microbial production of SA has been extensively investigated in Escherichia coli
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Open AccessHigh throughput sequencing of two celery varieties small RNAs identifies microRNAs involved in temperature stress response
MicroRNAs (miRNAs) are small, non-coding RNAs of 20 to 24 nucleotides that regulate gene expression and responses to biotic and abiotic stress. Till now, no reports have previously been published concerning mi...
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Open AccessComplete genome sequence and comparative genomic analyses of the vancomycin-producing Amycolatopsis orientalis
Amycolatopsis orientalis is the type species of the genus and its industrial strain HCCB10007, derived from ATCC 43491, has been used for large-scale production of the vital antibiotic vancomycin. However, to dat...
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Open AccessGenome organisation of the Acinetobacter lytic phage ZZ1 and comparison with other T4-like Acinetobacter phages
Phage ZZ1, which efficiently infects pathogenic Acinetobacter baumannii strains, is the fifth completely sequenced T4-like Acinetobacter phage to date. To gain a better understanding of the genetic characteristic...
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Open AccessGenome-wide association study for flowering time, maturity dates and plant height in early maturing soybean (Glycine max) germplasm
Soybean (Glycine max) is a photoperiod-sensitive and self-pollinated species. Days to flowering (DTF) and maturity (DTM), duration of flowering-to-maturity (DFTM) and plant height (PH) are crucial for soybean ada...
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Article
Open AccessGiniClust: detecting rare cell types from single-cell gene expression data with Gini index
High-throughput single-cell technologies have great potential to discover new cell types; however, it remains challenging to detect rare cell types that are distinct from a large population. We present a novel...
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Article
Open AccessEnhanced production of polyhydroxybutyrate by multiple dividing E. coli
Most bacteria are grown in a binary fission way meaning a bacterial cell is equally divided into two. Polyhydroxyalkanoates (PHA) can be accumulated as inclusion bodies by bacteria. The cell division way and m...
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Article
Open AccessChromosome engineering of Escherichia coli for constitutive production of salvianic acid A
Salvianic acid A (SAA), a valuable natural product from herbal plant Salvia miltiorrhiza, exhibits excellent antioxidant activities on food industries and efficacious therapeutic potential on cardiovascular disea...
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Open AccessAntimicrobial peptide gene BdPho responds to peptidoglycan infection and mating stimulation in oriental fruit fly, Bactrocera dorsalis (Hendel)
Phormicins belong to defensin family, which are important antimicrobial peptides (AMPs) in insects. These AMPs are inducible upon challenging by immune triggers. In the present study, we identified the cDNA of...
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Article
Open AccessTranscriptional reference map of hormone responses in wheat spikes
Phytohormones are key regulators of plant growth, development, and signalling networks involved in responses to diverse biotic and abiotic stresses. Transcriptional reference maps of hormone responses have bee...
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Open AccessIdentification of differentially expressed genes and pathways between intramuscular and abdominal fat-derived preadipocyte differentiation of chickens in vitro
The distribution and deposition of fat tissue in different parts of the body are the key factors affecting the carcass quality and meat flavour of chickens. Intramuscular fat (IMF) content is an important fact...
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Open AccessSoil pH is the primary factor driving the distribution and function of microorganisms in farmland soils in northeastern China
To understand which environmental factors influence the distribution and ecological functions of bacteria in agricultural soil.