5,172 Result(s)
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Article
Recent advances in anther culture of Hevea brasiliensis (Muell.-Arg.)
The yield of pollen embryoids from cultured Hevea anthers was increased 4 fold by optimizing the proportion of ammonium nitrate to potassium nitrate in the dedifferentiation medium. For optimal differentiation of...
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Article
Deoxycytidine reverses the suppression of nucleolar organizer regions' activity caused by BrdU
In this article we report that 5′-bromodeoxyuridine (BrdU) significantly suppresses the nucleolar organizer regions (NORs) activity of Chinese hamster cells (both dipliod cells and cell line Wg3-h) (P<0.001). One...
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Sister chromatid differential staining and NOR activity of BrdU-resistant sublines
Two 30 μg/ml BrdU-resistant sublines and two 60 μg/ml BrdU-resistant sublines are induced from a Chinese hamster cell line Wg3h (HGPRT−) by one-step and two-step selections, respectively. By inoculating the cells...
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Article
Establishment and characterization of a continuous murine uterine cervix cancer cell line metastatic to lymph nodes and lungs
The murine uterine cervix cancer (MUCC) cell line was derived from a chemically induced Kunming mouse uterine cervix cancer (U27) and maintained in culture on solid substrates for over 100 passages. Cultures w...
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Article
Spontaneous acquisition of tumorigenicity and invasiveness by mouse lens explant cells during culture in vitro
The lens of the eye is one of the rare organs in which tumors do not occur spontaneously. It therefore appeared to us that lens cells would not present the background of spontaneous transformation toward malig...
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Article
Role of interspecies transfer of chromosomal genes in the evolution of penicillin resistance in pathogenic and commensal Neisseria species
The two pathogenic species of Neisseria, N. meningitidis and N. gonorrhoeae, have evolved resistance to penicillin by alterations in chromosomal genes encoding the high molecular weight penicillin-binding protein...
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Article
Comparative analysis of glycoprotein and glycolipid composition of virulent and avirulent strain membranes ofMycoplasma hyopneumoniae
The glycoproteins and glycolipids from membranes of virulent strain Z and avirulent strain M ofMycoplasma hyopneumoniae have been compared. The proteins and the glycoproteins were identified by SDS-polyacrylamide...
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Article
Map** of the human integrin β7 gene (ITGβ7) to 12q13.13 by non-isotopic in situ hybridization
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Article
A DNA-binding protein factor in K562 nuclear extract interacts with positive control region (PCR) in the 5′-flanking sequence of human β-globin gene
It has been known that there are at least three regulatory regions (NCR1, NCR2 and PCR) in the 5′-flanking sequence (from −610 bp to +1 bp) of human β-globin gene and that the function of PCR is unique to the hum...
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Article
Establishment of somatic hybrid cell lines between Zea mays L. (maize) and Triticum sect, trititrigia MacKey (trititrigia)
Somatic hybrid cell lines were constructed by the fusion of protoplasts isolated from cell suspensions of Zea mays L. (maize, 2n = 20) and Triticum sect, trititrigia MacKey (trititrigia, 2n = 35), a perennial hyb...
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Article
Dry weight and chemical composition (CHN) in relation to population density of cultivatedTisbe holothuriae (Copepoda, Harpacticoida)
The harpacticoid copepodTisbe holothuriae was cultivated at different densities in a running-water system. Dry weight and chemical composition (CHN) of both females and egg-sacs have been determined. Dry weight d...
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Article
Effect of density on larval development and female productivity ofTisbe holothuriae (Copepoda, Harpacticoida) under laboratory conditions
The harpacticoid copepodTisbe holothuriae has been cultivated in the Helgoland laboratory for more than 20 years. The effects of density on the larval development and the female productivity were studied by compa...
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Article
Proteins binding to the 5′-flanking regulatory elements of the human β-globin gene
The binding of nuclear proteins prepared from mouse erythroid tissue in different developmental stages to the 5′-flanking regulatory elements of human β-globin gene, two negative control regions(NCR1, -610 to -49...
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Article
Effect of washing on the plasma membrane and on stress reactions of cultured rose cells
Cultured cells of Rosa damascena have been used as a model for studies of responses of plant cells to various stresses, including UV radiation, protein-synthesis inhibitors, and elicitors from pathogens. Many of ...
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Article
Assessing nitrogen mineralization from soil organic matter using anion exchange membranes
A simple method to assess differences in potential contribution of organic nitrogen mineralization to plant available N among soils may be useful in fertility research as well as routine soil testing. We deplo...
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Article
Factors affecting gibberellic acid production by Fusarium moniliforme in solid-state cultivation on starch
The production of gibberellic acid (GA3) by Fusarium moniliforme M-7121 in solid-state culture was evaluated in flask cultures as well as in 3-I horizontal rotary reactors. The highest production rate of GA3 was ...
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Article
RFLP-based phylogenetic analysis of wide compatibility varieties in Oryza sativa L.
Twenty-one wide compatibility varieties (WCVs) of rice together with three indica and three japonica testers were assayed with 160 DNA probes that were selected to represent the entire RFLP map at an average inte...
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Identification of the development stage-specific factors in mouse fetal liver binding to the human β-globin gene promoter
In order to elucidate the molecular mechanisms of globin gene expression during embryonic development, the nuclear extracts from mouse hematopoietic tissue at different stages of development have been prepared...
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Article
Resistance ofSclerotinia sclerotiorum to carbendazim
This paper covers the examination of EC50 of carbendazim to diferent isolates ofS. sclerotiorum, the observation of the resistance development in a population of the pathogen, for working out whether carbendazim ...
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Article
Study of a low-selenium environment in China by INAA and Mössbauer spectrometry
The neutron activation analysis, γ coincidence spectroscopy, nondispersive hydrogen flame atomic fluorescence spectroscopy, and Mössbauer spectrometry were used to study the low-selenium environment of the Exi...