15 Result(s)
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Article
David Schrama
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Open AccessMerkel Cell Carcinoma: Integrating Epidemiology, Immunology, and Therapeutic Updates
Merkel cell carcinoma (MCC) is a rare skin cancer characterized by neuroendocrine differentiation. Its carcinogenesis is based either on the integration of the Merkel cell polyomavirus or on ultraviolet (UV) m...
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Open AccessInhibition of p90 ribosomal S6 kinases disrupts melanoma cell growth and immune evasion
The mitogen-activated protein kinase (MAPK) signaling pathway is frequently hyperactivated in malignant melanoma and its inhibition has proved to be an efficient treatment option for cases harboring BRAFV600 muta...
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Open AccessMerkel cell carcinoma-derived exosome-shuttle miR-375 induces fibroblast polarization by inhibition of RBPJ and p53
Merkel cell carcinoma (MCC) is a highly invasive and metastatic skin cancer. While high expression of miR-375 is a characteristic of MCC, it seems not to contribute to the malignant phenotype of MCC cells. miR...
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Open AccessIFN-gamma-induced PD-L1 expression in melanoma depends on p53 expression
Immune checkpoint inhibition and in particular anti-PD-1 immunotherapy have revolutionized the treatment of advanced melanoma. In this regard, higher tumoral PD-L1 protein (gene name: CD274) expression is associa...
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Open AccessElucidating the mechanism of action of domatinostat (4SC-202) in cutaneous T cell lymphoma cells
Targeting epigenetic modifiers is effective in cutaneous T cell lymphoma (CTCL). However, there is a need for further improvement of this therapeutic approach. Here, we compared the mode of action of romidepsi...
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Open AccessEpigenetic priming restores the HLA class-I antigen processing machinery expression in Merkel cell carcinoma
Merkel cell carcinoma (MCC) is a rare and aggressive, yet highly immunogenic skin cancer. The latter is due to its viral or UV-associated carcinogenesis. For tumor progression MCC has to escape the host’s immu...
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Open AccessReversal of epigenetic silencing of MHC class I chain-related protein A and B improves immune recognition of Merkel cell carcinoma
Merkel cell carcinoma (MCC) is a virally associated cancer characterized by its aggressive behavior and strong immunogenicity. Both viral infection and malignant transformation induce expression of MHC class I...
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Open AccessDiscovery of CD8 T cell epitopes in Merkel cell polyomavirus through combinatorial encoding with MHC multimers
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Open AccessCellular and cytokine-dependent immunosuppressive mechanisms of grm1-transgenic murine melanoma
Grm1-transgenic mice spontaneously develop cutaneous melanoma. This model allowed us to scrutinize the generic immune responses over the course of melanoma development. To this end, lymphocytes obtained from s...
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Open AccessSurvivin-specific T-cell reactivity correlates with tumor response and patient survival: a phase-II peptide vaccination trial in metastatic melanoma
Therapeutic vaccination directed to induce an anti-tumoral T-cell response is a field of extensive investigation in the treatment of melanoma. However, many vaccination trials in melanoma failed to demonstrate...
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Open AccessImmunogenicity of HLA-A1-restricted peptides derived from S100A4 (metastasin 1) in melanoma patients
S100A4 (metastasin 1) belongs to the S100 family of Ca2+ binding proteins. While not present in most differentiated adult tissues, S100A4 is upregulated in the micromilieu of tumors. It is primarily expressed by ...
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Open AccessCancer treatment: the combination of vaccination with other therapies
Harnessing of the immune system by the development of ‘therapeutic’ vaccines, for the battle against cancer has been the focus of tremendous research efforts over the past two decades. As an illustration of th...
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Open AccessRelative quantification of TCR Vbeta-chain families by real time PCR for identification of clonal T-cell populations
Quantification of T-cell receptor (TCR) chain families can be utilized for detection of clonal T-cell populations. Besides southern blotting and antibody-based approaches, quantitative real time PCR (qRT PCR) ...
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Open AccessT-cell clonotypes in cancer
Cells of the immune system spontaneously recognize autologous tumor cells and T cells are believed to be the main effector cells for the immune surveillance of cancer. Recent advances in our understanding of b...
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Comparative delineation of T cell clonotypes in coexisting syngeneic B16 melanoma
B16 is a murine melanoma of C57Bl/6 origin, which rapidly develops as a tumor when inoculated into syngeneic immunocompetent hosts. Nevertheless, B16 tumors are considered to be immunogenic since tumor regres...
