117 Result(s)
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Article
Chunfang **
Life Sciences
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Article
Ball Tonometry: A Rapid, Nondestructive Method for Measuring Cell Turgor Pressure in Thin-Walled Plant Cells
In this article we describe a new method for the determination of turgor pressures in living plant cells. Based on the treatment of growing plant cells as thin-walled pressure vessels, we find that pressures c...
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Stochastic mitochondrial DNA changes: bioenergy decline in type I skeletal muscle fibres correlates with a decline in the amount of amplifiable full-length mtDNA
Extra-long PCR (XL-PCR) was used to assess the relative concentration of functional full-length mitochondrial DNA (mtDNA) in single type I human vastus lateralis muscle fibres of defined cytochrome c oxidase (COX...
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Loss of stability, pH, and the anisotropic extensibility of Chara cell walls
We investigated the effects of acid conditions on the extensibility of isolated wall segments from growing Chara corallina cells, providing the first detailed multi-azimuthal description of the anisotropic elasti...
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Cloning and expression of Mycobacterium bovis secreted protein MPB51 in Escherichia coli
The purpose of this study is to clone, identify, and express the mature secreted protein MPB51 from Mycobacterium bovis and to lay a good foundation for the diagnosis of M. bovis, for applying M. bovis vaccine in...
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Identifying cancer genes from cancer mutation profiles by cancer functions
It is of great importance to identify new cancer genes from the data of large scale genome screenings of gene mutations in cancers. Considering the alternations of some essential functions are indispensable fo...
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Hyaluronic acid fragments evoke Kupffer cells via TLR4 signaling pathway
Kupffer cells, expressing toll-like receptor 4 (TLR4), play a central role in hepatic ischemia/reperfusion (I/R) injury. Hyaluronic acid (HA) fragments, degradative products of high-molecular-weight HA (HMW-HA...
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Open AccessMultichromosomal median and halving problems under different genomic distances
Genome median and genome halving are combinatorial optimization problems that aim at reconstructing ancestral genomes as well as the evolutionary events leading from the ancestor to extant species. Exploring c...
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Dynamic changes in Tangxunhu wetland over a period of rapid development (1953–2005) in Wuhan, China
Tangxunhu wetland is one of China’s largest freshwater lakes and plays a significant role in the sustainable development of the city of Wuhan. Based on terrain maps, TM images, and statistical data from 1953 t...
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Laboratory evaluation of a walleye (Sander vitreus) bioenergetics model
Walleye (Sander vitreus) is an important game fish throughout much of North America. We evaluated the performance of the Wisconsin bioenergetics model for walleye in the laboratory. Walleyes were fed rainbow smel...
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Open AccessThe collapse of gene complement following whole genome duplication
Genome amplification through duplication or proliferation of transposable elements has its counterpart in genome reduction, by elimination of DNA or by gene inactivation. Whether loss is primarily due to excis...
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Soluble expression and purification of the anthrax protective antigen in E. coli and identification of a novel dominant-negative mutant N435C
The anthrax toxin is an AB-type bacterium toxin composed of the protective antigen (PA) as the cell-binding B component, and the lethal factor (LF) and edema toxin (EF) as the catalytic A components. The PA co...
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Open AccessScaffold filling, contig fusion and comparative gene order inference
There has been a trend in increasing the phylogenetic scope of genome sequencing without finishing the sequence of the genome. Increasing numbers of genomes are being published in scaffold or contig form. Rear...
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Proteome expression patterns in the stress tolerant evergreen Ammopiptanthus nanus under conditions of extreme cold
Low temperature is one of the important environmental changes that affect plant growth. The cold resistance capabilities of evergreen plants are the result of long-term adaptation to extreme environmental cond...
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Open AccessPopulation genetics of foxtail millet and its wild ancestor
Foxtail millet (Setaria italica (L.) P. Beauv.), one of the most ancient domesticated crops, is becoming a model system for studying biofuel crops and comparative genomics in the grasses. However, knowledge on th...
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Open AccessOn the PATHGROUPS approach to rapid small phylogeny
We present a data structure enabling rapid heuristic solution to the ancestral genome reconstruction problem for given phylogenies under genomic rearrangement metrics. The efficiency of the greedy algorithm is...
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Open AccessComparative transcriptome sequencing of germline and somatic tissues of the Ascaris suum gonad
Ascaris suum (large roundworm of pigs) is a parasitic nematode that causes substantial losses to the meat industry. This nematode is suitable for biochemical studies because, unlike C. elegans, homogeneous tissue...
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Open AccessFractionation statistics
Paralog reduction, the loss of duplicate genes after whole genome duplication (WGD) is a pervasive process. Whether this loss proceeds gene by gene or through deletion of multi-gene DNA segments is controversi...
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Open AccessA model for biased fractionation after whole genome duplication
Paralog reduction, the loss of duplicate genes after whole genome duplication (WGD) is a pervasive process. Whether this loss proceeds gene by gene or through deletion of multi-gene DNA segments is controversi...
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An intronic polymorphism rs2237062 in the CXCL14 gene influences HBV-related HCC progression in Chinese population
CXCL14 (C-X-C motif chemokine ligand 14) is a conserved member of chemokine family and functions as a chemoattractant for multiplicate immunocytes. CXCL14 expression is constitutive in no...
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Open AccessGene order in rosid phylogeny, inferred from pairwise syntenies among extant genomes
Ancestral gene order reconstruction for flowering plants has lagged behind developments in yeasts, insects and higher animals, because of the recency of widespread plant genome sequencing, sequencers' embargoe...
