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  1. No Access

    Article

    A Novel Mucosal Vaccine Against Foot-and-Mouth Disease Virus Induces Protection in Mice and Swine

    Epitopes of a foot-and-mouth disease virus (FMDV) capsid protein VP1 complex and a chimera of 6×His-tagged cholera toxin B subunit (hCTB) were expressed in Hansenula polymorpha and used together as a mucosal vacc...

    Houhui Song, Zhiliang Wang, Dongxia Zheng, Weihuan Fang, Yong Li… in Biotechnology Letters (2005)

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    Article

    Design of Vectors for Efficient Integration and Transformation in Hansenula polymorpha

    Four vectors were constructed for high-level expression of heterologous proteins with high copy number and mitotic stability in Hansenula polymorpha. All of them contained the conserved H. polymorpha-derived ribo...

    Yueyong Liu, Yong Li, Liyue Liu, Xuebo Hu, Bingsheng Qiu in Biotechnology Letters (2005)

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    Article

    Detection of foot-and-mouth virus antibodies using a purified protein from the high-level expression of codon-optimized, foot-and-mouth disease virus complex epitopes in Escherichia coli

    A codon optimized DNA sequence coding for foot-and-mouth disease virus (FMDV) capsid protein complex epitopes of VP1 amino acid residues 21–40, 135–160, and 200–213 was genetically fused to the C-terminal end of ...

    Houhui Song, Weihuan Fang, Zhiliang Wang, Dongxia Zheng, Jian Du… in Biotechnology Letters (2004)

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    Article

    Expression of a human lactoferrin N-lobe in Nicotiana benthmiana with potato virus X-based agroinfection

    A DNA fragment encoding the N-terminal half (N-lobe) of the human lactoferrin (hLfN) gene has now been cloned into recombinant Potexvirus potato virus X (PVX) vector and expressed in Nicotiana benthmiana using ag...

    Yong Li, Yunfeng Geng, Houhui Song, Guangyu Zheng, Liandong Huan… in Biotechnology Letters (2004)

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    Article

    Development of a set of expression vectors in Hansenula polymorpha

    Four expression vectors based on formate dehydrogenase promoter (FMDp) and methanol oxidase promoter (MOXp) from Hansenula polymorpha were developed to express heterologous genes in Hansenula polymorpha. A secret...

    Houhui Song, Yong Li, Weihuan Fang, Yunfeng Geng, Xu Wang… in Biotechnology Letters (2003)