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  1. No Access

    Article

    Expression and use of the green fluorescent protein as a reporter system in Legionella pneumophila

    The gene encoding the green fluorescent protein (GFP) was used as a reporter gene in Legionella pneumophila. To analyze GFP expression in Legionella, transcriptional fusions of gfp with the Legionella-specific m...

    R. Köhler, A. Bubert, W. Goebel, M. Steinert… in Molecular and General Genetics MGG (2000)

  2. No Access

    Article

    Differential expression of Listeria monocytogenes virulence genes in mammalian host cells

    We have used RT-PCR and GFP-mediated fluorescence to analyse the regulation of PrfA-dependent virulence genes of Listeria monocytogenes during proliferation in mammalian host cells. Our data show that most of th...

    A. Bubert, Z. Sokolovic, S.-K. Chun, L. Papatheodorou… in Molecular and General Genetics MGG (1999)

  3. No Access

    Article

    The gene cluster inlC2DE of Listeria monocytogenes contains additional new internalin genes and is important for virulence in mice

    In this work we identified and characterized a gene cluster containing three internalin genes of Listeria monocytogenes EGD. These genes, termed inlG, inlH and inlE, encode proteins of 490, 548 and 499 amino aci...

    D. Raffelsbauer, A. Bubert, F. Engelbrecht… in Molecular and General Genetics MGG (1998)

  4. No Access

    Article

    The Listeria monocytogenesiap gene as an indicator gene for the study of PrfA-dependent regulation

    The iap gene of Listeria monocytogenes encodes the extracellular protein p60, which possesses a murein hydrolase activity necessary for septum separation. We constructed L. monocytogenes EGD strains harbouring p...

    A. Bubert, H. Kestler, M. Götz, R. Böckmann… in Molecular and General Genetics MGG (1997)