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  1. Article

    Open Access

    Genome editing toward biofortified soybean with minimal trade-off between low phytic acid and yield

    Phytic acid (PA) in grain seeds reduces the bioavailability of nutrient elements in monogastric animals, and an important objective for crop seed biofortification is to decrease the seed PA content. Here, we e...

    Wenxin Lin, Mengyan Bai, Chunyan Peng, Huaqin Kuang, Fanjiang Kong… in aBIOTECH (2024)

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  2. Article

    Open Access

    A sensitive one-pot ROA assay for rapid miRNA detection

    MicroRNAs (miRNAs) and short RNA fragments (18–25 nt) are crucial biomarkers in biological research and disease diagnostics. However, their accurate and rapid detection remains a challenge, largely due to thei...

    Zhihao Hou, Wenpeng Deng, Alun Li, Ya Zhang, Jianye Chang, **nyue Guan… in aBIOTECH (2024)

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  3. Article

    Open Access

    Elimination of an unfavorable allele conferring pod shattering in an elite soybean cultivar by CRISPR/Cas9

    Pod shattering can lead to devastating yield loss of soybean and has been a negatively selected trait in soybean domestication and breeding. Nevertheless, a significant portion of soybean cultivars are still p...

    Zhihui Zhang, Jie Wang, Huaqin Kuang, Zhihong Hou, **** Gong, Mengyan Bai… in aBIOTECH (2022)

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  4. No Access

    Article

    Genome-Wide Comparative Analysis of Genetic Diversity of Regular and Specialty Maize Inbred Lines Through Genoty** by Target Sequencing (GBTS)

    Specialty maize is widely used for human and animal consumption. It is therefore important to conduct a genome-wide comparison of regular and specialty maize. In the present study, a total of 20,000 SNP marker...

    Diansi Yu, Lili Song, Wei Gu, Yuan Guan, Hui Wang… in Plant Molecular Biology Reporter (2022)

  5. Article

    Open Access

    Global Analysis of Gene Expression Profiles in Glutinous Rice 89-1 (Oryza sativa L.) Seedlings Exposed to Chilling Stress

    Rice (Oryza sativa) is a tropical cereal crop that is severely affected by chilling stress at the seedling stage, although glutinous rice 89-1 (Gr89-1) in Chongqing, China, shows tolerance to low temperatures and...

    **aoxue Pan, Hong Wu, Mingyu Hu, Zhongwei Wang… in Plant Molecular Biology Reporter (2021)

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  6. No Access

    Article

    Interplay between the shoot apical meristem and lateral organs

    Tissues and organs within a living organism are coordinated, but the underlying mechanisms are not well understood. The shoot apical meristem (SAM) continually produces lateral organs, such as leaves, from its...

    Chunmei Guan, Yuling Jiao in aBIOTECH (2020)

  7. No Access

    Article

    Cloning and Characterization of a gene Encoding True D-cysteine Desulfhydrase from Oryza sativa

    Hydrogen sulfide (H2S) has been regarded as the third gasotransmitter and plays an active role in multiple signaling events of plants and animals. Cysteine desulfhydrases (CDes), including both D- and L-cysteine ...

    Heng Zhou, Wenxue Guan, Mingjian Zhou, Jie Shen… in Plant Molecular Biology Reporter (2020)

  8. No Access

    Article

    Comparative Transcriptome Profile Analysis of Anther Development in Reproductive Stage of Rice in Cold Region Under Cold Stress

    Rice in cold region is threatened by cold stress frequently, resulting in severe losses of rice production. In the present study, two rice varieties with contrast cold tolerance in the reproductive stage (Long...

    Zhenhua Guo, Chuanxue Liu, Wuming **ao, Ruiying Wang… in Plant Molecular Biology Reporter (2019)

  9. No Access

    Article

    Genetic Dissection of Wheat Kernel Hardness Using Conditional QTL Map** of Kernel Size and Protein-Related Traits

    Kernel hardness (KH) is one of the primary quality parameters for common wheat (Triticum aestivum L.) and has a major impact on milling, flour quality, and end-product properties. In addition to Puroindoline (Pin

    **aoxiao Sun, Tongtong Liu, Tangyuan Ning, Kai Liu… in Plant Molecular Biology Reporter (2018)

  10. No Access

    Article

    Anthocyanin Biosynthesis Regulation in the Fruit of Citrus sinensis cv. Tarocco

    The differential expression profiling of the fruit-specific genes between a blood orange (Citrus sinensis cv. Tarocco) and a blonde orange (Citrus sinensis cv. twenty-first century navel orange) was investigated ...

    Jian-hui Wang, Jian-jun Liu, Ke-ling Chen, Hong-wen Li… in Plant Molecular Biology Reporter (2016)

  11. No Access

    Article

    Molecular Characterization of Ethylene-Regulated Anthocyanin Biosynthesis in Plums During Fruit Ripening

    Anthocyanin accumulation is an important physiological process that occurs during plum fruit ripening. Currently, little is known about the molecular mechanisms of ethylene-regulated anthocyanin accumulation i...

    Yudou Cheng, Liqin Liu, Can Yuan, Junfeng Guan in Plant Molecular Biology Reporter (2016)

  12. No Access

    Article

    Isolation and Characterization of a SOC1-Like Gene from Tree Peony (Paeonia suffruticosa)

    Tree peony (Paeonia suffruticosa) is one of the well-known ornamental plants, and flowering is a major industry in its production areas, but its dormancy is a major obstacle during winter. Analysis of the molecul...

    Yuxi Zhang, YuE Li, Yang Zhang, Shiming Guan… in Plant Molecular Biology Reporter (2015)

  13. No Access

    Article

    Isolation and Characterization of IaYABBY2 Gene from Incarvillea arguta

    The establishment of ad-abaxial polarity is an important characteristic of the development of lateral organs in plants. YABBY genes encode higher plant-specific nuclear proteins which play critical roles in pr...

    Xudong Sun, Yanlong Guan, **angyang Hu in Plant Molecular Biology Reporter (2014)

  14. No Access

    Article

    Isolation and Characterization of a Lipid Transfer Protein Gene (BplLTP1) from Betula platyphylla

    Although nonspecific lipid transfer proteins (nsLTPs) are widely present in plants, their functions are not fully understood. Here, we isolated and characterized a putative nsLTP gene, BplLTP1, from Betula platyp...

    Minxiao Guan, Ruihai Chai, Xue Kong, Xuemei Liu in Plant Molecular Biology Reporter (2013)

  15. No Access

    Article

    Overexpression of AtchyB in Eustoma grandiflorum Shinn Enhances its Tolerance to High-Light Via Zeaxanthin Accumulation

    Carotenoids are essential components of the photosynthetic apparatus involved in plant photoprotection. To investigate the protective role of zeaxanthin and the xanthophyll cycle under high-light stress, we in...

    Weidang Wu, **g Ji, Gang Wang, Qing Zhao, Chao **… in Plant Molecular Biology Reporter (2012)

  16. No Access

    Article

    Isolation and Characterization of a Temperature-Regulated Microsomal Oleate Desaturase Gene (CtFAD2-1) from Safflower (Carthamus tinctorius L.)

    A cDNA sequence, designated CtFAD2-1 and putatively encoding a delta-12 fatty acid desaturase, was isolated from total RNA of immature normal-type safflower (Carthamus tinctorius L.) seeds using a PCR approach. T...

    Ling-Liang Guan, Ying-Wen Xu, Yuan-Biao Wang, Li Chen… in Plant Molecular Biology Reporter (2012)

  17. No Access

    Article

    Molecular Cloning and Expression Analysis of Genes Encoding Two Microsomal Oleate Desaturases (FAD2) from Safflower (Carthamus tinctorius L.)

    The endoplasmic reticulum (ER)-associated oleate desaturase (FAD2) is the key enzyme responsible for the production of linoleic acid in plants. In safflower, a seed-type FAD2 gene (CtFAD2-1) has been isolated pre...

    Ling-Liang Guan, Yuan-Biao Wang, Hao Shen, Kai Hou… in Plant Molecular Biology Reporter (2012)

  18. No Access

    Article

    Efficient shoot regeneration andAgrobacterium-mediated transformation ofBrassica juncea

    Procedures for callus induction, plantlet regeneration, andAgrobacterium-mediated transformation ofBrassica juncea were optimized by studying several factors, including explant types, and various plant growth reg...

    Zhang Yuxiu, Xu **, Han Lu, Wei Wei, Guan Ziqiu… in Plant Molecular Biology Reporter (2006)

  19. No Access

    Article

    PCR-enriched cDNA pool method for cloning of gene homologues

    We have developed a PCR-enriched cDNA pooling method for enrichment of the complete 5′ ends of the target homology cDNA fragments with just 1 conserved region needed. By using reverse transcription and a few r...

    Minglin Lang, Yuxiu Zhang, Ziqiu Guan, Tuanyao Chai in Plant Molecular Biology Reporter (2005)