26 Result(s)
within
English
Life Sciences
Agriculture
Bioinformatics
**fa Guan
Article
-
Article
Open AccessCrowd-sourced benchmarking of single-sample tumor subclonal reconstruction
Subclonal reconstruction algorithms use bulk DNA sequencing data to quantify parameters of tumor evolution, allowing an assessment of how cancers initiate, progress and respond to selective pressures. We launc...
-
Article
Author Correction: Adenine transversion editors enable precise, efficient A•T-to-C•G base editing in mammalian cells and embryos
-
Article
Author Correction: Re-engineering the adenine deaminase TadA-8e for efficient and specific CRISPR-based cytosine base editing
-
Article
Open AccessGenome editing toward biofortified soybean with minimal trade-off between low phytic acid and yield
Phytic acid (PA) in grain seeds reduces the bioavailability of nutrient elements in monogastric animals, and an important objective for crop seed biofortification is to decrease the seed PA content. Here, we e...
-
Article
Adenine transversion editors enable precise, efficient A•T-to-C•G base editing in mammalian cells and embryos
Base editors have substantial promise in basic research and as therapeutic agents for the correction of pathogenic mutations. The development of adenine transversion editors has posed a particular challenge. H...
-
Article
Open AccessA sensitive one-pot ROA assay for rapid miRNA detection
MicroRNAs (miRNAs) and short RNA fragments (18–25 nt) are crucial biomarkers in biological research and disease diagnostics. However, their accurate and rapid detection remains a challenge, largely due to thei...
-
Article
Re-engineering the adenine deaminase TadA-8e for efficient and specific CRISPR-based cytosine base editing
Cytosine base editors (CBEs) efficiently generate precise C·G-to-T·A base conversions, but the activation-induced cytidine deaminase/apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like (AID/APOBEC)...
-
Article
Open AccessElimination of an unfavorable allele conferring pod shattering in an elite soybean cultivar by CRISPR/Cas9
Pod shattering can lead to devastating yield loss of soybean and has been a negatively selected trait in soybean domestication and breeding. Nevertheless, a significant portion of soybean cultivars are still p...
-
Article
A fluorescent sensor for spatiotemporally resolved imaging of endocannabinoid dynamics in vivo
Endocannabinoids (eCBs) are retrograde neuromodulators with important functions in a wide range of physiological processes, but their in vivo dynamics remain largely uncharacterized. Here we developed a geneti...
-
Article
Identifying phenotype-associated subpopulations by integrating bulk and single-cell sequencing data
Bulk and single cell measurements are integrated to identify phenotype-associated subpopulations of cells.
-
Article
A small and highly sensitive red/far-red optogenetic switch for applications in mammals
Optogenetic technologies have transformed our ability to precisely control biological processes in time and space. Yet, current eukaryotic optogenetic systems are limited by large or complex optogenetic module...
-
Article
Toward best practice in cancer mutation detection with whole-genome and whole-exome sequencing
Clinical applications of precision oncology require accurate tests that can distinguish true cancer-specific mutations from errors introduced at each step of next-generation sequencing (NGS). To date, no bulk ...
-
Article
Interplay between the shoot apical meristem and lateral organs
Tissues and organs within a living organism are coordinated, but the underlying mechanisms are not well understood. The shoot apical meristem (SAM) continually produces lateral organs, such as leaves, from its...
-
Article
Publisher Correction: The Extended Polydimensional Immunome Characterization (EPIC) web-based reference and discovery tool for cytometry data
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
-
Article
The Extended Polydimensional Immunome Characterization (EPIC) web-based reference and discovery tool for cytometry data
-
Article
A community effort to create standards for evaluating tumor subclonal reconstruction
Tumor DNA sequencing data can be interpreted by computational methods that analyze genomic heterogeneity to infer evolutionary dynamics. A growing number of studies have used these approaches to link cancer ev...
-
Article
Dimensionality reduction for visualizing single-cell data using UMAP
A benchmarking analysis on single-cell RNA-seq and mass cytometry data reveals the best-performing technique for dimensionality reduction.
-
Article
Prediction of potent shRNAs with a sequential classification algorithm
The most effective shRNAs to silence a gene are calculated by a machine learning algorithm.
-
Article
A time and a place for sugar in your ears
Targeting trehalose metabolism improves maize yield under a range of water-deficit conditions.
-
Article
Open AccessSequencing of allotetraploid cotton (Gossypium hirsutum L. acc. TM-1) provides a resource for fiber improvement
Two draft sequences of Gossypium hirsutum, the most widely cultivated cotton species, provide insights into genome structure, genome rearrangement, gene evolution and cotton fiber biology.
