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Bovid Interspecies Somatic Cell Nuclear Transfer with Ooplasm Transfer
Interspecies somatic cell nuclear transfer (iSCNT) contributes to the preservation of endangered species, albeit nuclear–mitochondrial...
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Horse Somatic Cell Nuclear Transfer Using Zona Pellucida-Enclosed and Zona-Free Oocytes
Horse cloning by somatic cell nuclear transfer (SCNT) is an attractive scientific and commercial endeavor. Moreover, SCNT allows generating...
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Somatic Cell Nuclear Transfer in Rabbits
Somatic cell nuclear transfer (SCNT) is a technology that enables differentiated somatic cells to acquire a totipotent state, thus making it of great...
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An Automated Strategy to Handle Antigenic Variability in Immunisation Protocols, Part I: Nanopore Sequencing of Infectious Agent Variants
Infectious agents often challenge therapeutics, from antibiotics resistance to antigenic variability affecting inoculation measures. Over the last...
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In Vitro Assay for the Assessment of Oxygen Depletion Triggers in Human Cell Lines, Associated with Improving Responses to Cancer Therapy
Tumors are usually associated with oxygen-deficient regions (hypoxia) which results from reduced and disorganized intratumoral vasculature, increased...
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Latest Trends in Nucleic Acids’ Engineering Techniques Applied to Precision Medicine
Nucleic acids are paving the way for advanced therapeutics. Unveiling the genome enabled a better understanding of unique genotype–phenotype...
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Simplified CRISPR-Mediated DNA Editing in Multicellular Eukaryotes
The CRISPR-Cas9 system is becoming an imperative tool to edit the genome of various organisms. The gene-editing study by the CRISPR-Cas9 system has...
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Cloning by SCNT: Integrating Technical and Biology-Driven Advances
Somatic cell nuclear transfer (SCNT) into enucleated oocytes initiates nuclear reprogramming of lineage-committed cells to totipotency. Pioneer SCNT...
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An Automated Strategy to Handle Antigenic Variability in Immunisation Protocols, Part II: In Vitro Transcribed mRNA Vector Design for Inoculation Against Infectious Agent Variants
A fully automated strategy to handle antigenic variability in immunisation protocols is here presented. The method comprises of (1) nanopore...
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Cartilage Tissue Engineering: An Introduction
Once damaged, cartilage has limited healing capability. This has led to a huge body of research that aims to repair or regenerate this important...
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Articular Cartilage Imaging in the Context of the Superficial Chondrocyte Spatial Organization (SCSO) as a Surrogate Marker for Functional Pathology
Articular cartilage imaging has undergone tremendous changes and nowadays enables functionally relevant quantitative information useful for detecting...
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Cattle Cloning by Somatic Cell Nuclear Transfer
Cloning by somatic cell Nuclear Transfer (SCNT) is a powerful technology capable of reprograming terminally differentiated cells to totipotency for...
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Derivation of Bovine Primed Embryonic Stem Cells from Somatic Cell Nuclear Transfer Embryos
Derivation of bovine embryonic stem cells from somatic cell nuclear transfer embryos enables the derivation of genetically matched pluripotent stem...
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Somatic Cell Nuclear Transfer Using Freeze-Dried Protaminized Donor Nuclei
Somatic cell nuclear transfer (SCNT) is the only nuclear reprogramming method that allows rewinding an adult nucleus into a totipotent state. As...
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Mitochondrial Inheritance Following Nuclear Transfer: From Cloned Animals to Patients with Mitochondrial Disease
Mitochondria are indispensable power plants of eukaryotic cells that also act as a major biochemical hub. As such, mitochondrial dysfunction, which...
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The Use of 99mTc-Labeled Stem Cells for Evaluation of Chronic Kidney Disease
The labeling of stem cells with radionuclides allows in vivo monitoring of cell migration and homing. Here, we describe the labeling of mononuclear...
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A Brief Review of Plant Cell Transfection, Gene Transcript Expression, and Genotypic Integration for Enhancing Compound Production
Plants possess a plethora of important secondary metabolites, which are unique sources of natural pigments, pharmaceutical compounds, food additives,...
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Stem Cell-Derived Microfluidic Amniotic Sac Embryoid (μPASE)
The microfluidic amniotic sac embryoid (μPASE) is a human pluripotent stem cell (hPSC)-derived multicellular human embryo-like structure with...
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Inferring Gene Regulatory Networks and Predicting the Effect of Gene Perturbations via IQCELL
IQCELL is a platform that infers Boolean gene regulatory networks from single-cell RNA sequencing data. Boolean networks can be simulated under...
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Guinea Pig Preimplantation Embryos: Generation, Collection, and Immunofluorescence
Studying various animal models is important for comparative biology and to better understand evolutionary development. Furthermore, when aiming to...