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Latest Trends in Nucleic Acids’ Engineering Techniques Applied to Precision Medicine

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Gene, Drug, and Tissue Engineering

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2575))

Abstract

Nucleic acids are paving the way for advanced therapeutics. Unveiling the genome enabled a better understanding of unique genotype–phenotype profiling. Methods for engineering and analysis of nucleic acids, from polymerase chain reaction to Cre-Lox recombination, are contributing greatly to biomarkers’ discovery, map** of cellular signaling cascades, and smart design of therapeutics in precision medicine. Investigating the different subtypes of DNA and RNA via sequencing and profiling is empowering the scientific community with valuable information, to be used in advanced therapeutics, tracking epigenetics linked to disease. Recent results from the application of nucleic acids in novel therapeutics and precision medicine are very encouraging, demonstrating great potential to treat cancer, viral infections via inoculation (e.g., SAR-COV-2 mRNA vaccines), along with metabolic and genetic disorders. Limitations posed by challenges in delivery mode are being addressed to enable efficient guided-gene-programmed precision therapies. With the focus on genetic engineering and novel therapeutics, more precisely, in precision medicine, this chapter discusses the advance enabled by knowledge derived from these innovative branches of biotechnology.

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  1. Bioengineering Department, Imperial College London, London, UK

    Glaucia C. Pereira

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    Glaucia C. Pereira

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Pereira, G.C. (2023). Latest Trends in Nucleic Acids’ Engineering Techniques Applied to Precision Medicine. In: Pereira, G.C. (eds) Gene, Drug, and Tissue Engineering. Methods in Molecular Biology, vol 2575. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2716-7_2

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  • DOI: https://doi.org/10.1007/978-1-0716-2716-7_2

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