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Protocol for the Generation of Human EPS-Blastoids Using a Three-Dimensional Two-Step Induction System
Stem cell-derived embryos in vitro allow the exploration of the very early stages of human embryogenesis in vitro and are thus promising for...
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ShapeMetrics: A 3D Cell Segmentation Pipeline for Single-Cell Spatial Morphometric Analysis
There is a growing need for single-cell level data analysis in correlation with the advancements of microscopy techniques. Morphology-based...
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Generation of 3D Trophoblast Organoids from Human Naïve Pluripotent Stem Cells
The human placenta is a transient organ that functions to support the needs of the fetus throughout gestation. Trophoblasts are the major epithelial...
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Embryonic Spinal Cord Innervation in Human Trunk Organogenesis Gastruloids: Cardiac Versus Enteric Customization and Beyond
Trunk-biased human gastruloids provide the ability to couple developmentally relevant spinal neurogenesis and organ morphogenesis via spatiotemporal...
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Use of Epigenetic Cues and Mechanical Stimuli to Generate Blastocyst-Like Structures from Mammalian Skin Dermal Fibroblasts
Mammalian embryogenesis is characterized by complex interactions between embryonic and extra-embryonic tissues that coordinate morphogenesis,...
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Whole-Mount RNA, Single-Molecule RNA (smRNA), and DNA Fluorescence In Situ Hybridization (FISH) in Mammalian Embryos
Fluorescence in situ hybridization (FISH) provides a valuable tool for studying the spatial localization of and expression level of genes and cell...
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Platelet-Rich Plasma for Intra-articular Injections: Preclinical and Clinical Evidence
The use of platelet-rich plasma (PRP) has been supported by encouraging data from in vitro and preclinical in vivo studies, both in terms of safety...
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Stem Cells as Nuclear Donors for Mammalian Cloning
Mammals are routinely cloned by introducing somatic nuclei into enucleated oocytes. Cloning contributes to propagating desired animals, to germplasm...
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Assessing the Challenges of Nanotechnology-Driven Targeted Therapies: Development of Magnetically Directed Vectors for Targeted Cancer Therapies and Beyond
Targeted delivery, in which therapeutic agents are preferentially concentrated at the diseased site, has the potential to improve therapeutic...
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Advances in Regenerative Medicine and Biomaterials
The low regenerative potential of the human body hinders proper regeneration of dysfunctional or lost tissues and organs due to trauma, congenital...
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Co-culture of Human Articular Chondrocytes Seeded in Polyurethane Scaffolds and Human Mesenchymal Stromal Cells Encapsulated in Alginate Beads
Co-culturing is an essential method for unravelling the importance of cross talk and cellular interaction. This chapter describes the preparation of...
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Purification and Isolation of Proteins from Hyaline Cartilage
Proteins from hyaline or articular cartilage can be isolated and purified using a series of chemical extraction steps and various identification...
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Metabolomic Profiling to Understand Chondrocyte Metabolism
Metabolism has long been recognized as a critical physiological process necessary to maintain homeostasis in all types of cells including the...
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Osteochondral Explant Isolation and Culture Under a Compression and Shear Bioreactor
Osteochondral explants harvested from different species are valuable preclinical ex vivo models for tissue engineering research. In this chapter, we...
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Mouse Cloning Using Outbred Oocyte Donors and Nontoxic Reagents
Somatic cell nuclear transfer (SCNT) technology has become a useful tool for animal cloning, gene manipulation, and genomic reprogramming research....
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Production of Cloned Pigs by Handmade Cloning
Somatic cell nuclear transfer (SCNT) in pigs is a promising technology in biomedical research by association with transgenesis for...
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Mass Spectroscopy as an Analytical Tool to Harness the Production of Secondary Plant Metabolites: The Way Forward for Drug Discovery
The molecular map of diverse biological molecules linked with structure, function, signaling, and regulation within a cell can be elucidated using an...
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Decellularization of Articular Cartilage: A Hydrochloric Acid–Based Strategy
Removing cellular material from a tissue, a process called decellularization, reduces the risk of adverse host reactions, allows for efficient...
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Isolation of Chondrons from Hyaline Cartilage
In native healthy hyaline cartilage, the chondrocytes are surrounded by a pericellular matrix that has a distinct composition and function compared...