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Synthetic Biology to Engineer Bacteriophage Genomes
Recent advances in the synthetic biology field have enabled the development of new molecular biology techniques used to build specialized...
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Rapid Bench to Bedside Therapeutic Bacteriophage Production
It has been over 100 years since bacteriophages (phages) were used as a human therapeutic. Since then, phage production has dramatically evolved....
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Nano/microformulations for Bacteriophage Delivery
Encapsulation methodologies allow the protection of bacteriophages for overcoming critical environmental conditions. Moreover, they improve the...
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A Review of Phage Therapy for Bone and Joint Infections
There is a strong rationale for using phages in patients with bone and joint infections (BJIs). Indeed, specific phages can infect and replicate in...
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The Oxford Nanopore MinION as a Versatile Technology for the Diagnosis and Characterization of Emerging Plant Viruses
The emergence of novel viral epidemics that could affect major crops represents a serious threat to global food security. The early and accurate...
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Discovery and Identification of Viruses Infecting Oomycetes
This chapter describes protocols suitable for the detection and identification of RNA viruses infecting oomycetes (so-called water molds of Kingdom...
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Detection of Plant Viruses Using Nanopore Sequencing Based Metagenomic Approach
Nanopore sequencing has proven to be a useful tool for the generic detection of plant viruses, especially in laboratories working with small number...
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Validation of High-Throughput Sequencing (HTS) for Routine Detection of Citrus Viruses and Viroids
The management of plant diseases relies on the accurate identification of pathogens that requires a robust and validated tool in terms of...
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Determination of the Mycovirome of a Necrotrophic Fungus
Next-generation sequencing (NGS) of total RNA has allowed the detection of novel viruses infecting different hosts, such as fungi, increasing our...
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Rescue of Infectious Salmon Anemia Virus (ISAV) from Cloned cDNA
The piscine orthomyxovirus called infectious salmon anemia virus (ISAV) is one of the most important emerging pathogens affecting the salmon industry...
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Reverse Genetics System for Rift Valley Fever Virus
Rift Valley fever virus (RVFV) is an important mosquito-borne virus that can cause severe disease manifestations in humans including ocular damage,...
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Reverse Genetics Systems for Filoviruses
Filoviruses are causative agents of severe hemorrhagic fevers with high case fatality rates in humans. For studies of virus biology and the...
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One-Step Purification Strategy for Cowpea Chlorotic Mottle Virus-Like Particles Produced by the IC-BEVS
Virus-like particles (VLP) of the cowpea chlorotic mottle virus (CCMV), a plant virus, have been shown to be safe and noncytotoxic vehicles for...
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Recombinant AAV Purification
Purification of rAAV is a crucial unit operation of the AAV production process. It enables the capture of AAV and removal of contaminants such as...
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Extraction of Modified Adeno-Associated Virus-Like Particles Displaying Peptides on Their Surface
Virus-like particles (VLPs) of the adeno-associated virus (AAV) can be produced using the baculovirus expression vector system. Insertion of small...
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Construction of Recombinant Baculoviruses
The success of using the insect cell-baculovirus expression technology (BEST) relies on the efficient construction of recombinant baculovirus with...
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Assembly of Baculovirus Vectors for Multiplexed Prime Editing
Efficient genome editing by using CRISPR technologies requires simultaneous and efficient delivery of multiple genetically encoded components to...
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Optimized Workflow from Gene to Product
This chapter outlines the workflow using the ExpiSf™ Expression System designed for high-density infection of suspension ExpiSf9™ cells. The system...
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Generation of Complex Protein Structures by Coinfection with High-Quality Recombinant Baculovirus
The baculovirus expression vector system (BEVSbaculovirus expression vector system (BEVS))Baculovirus expression vector system (BEVS) is a powerful...
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Production of Recombinant Viral Antigens Using the Baculovirus-Insect Cell Expression System
Insect cell expression has been successfully used for the production of viral antigens as part of commercial vaccine development. As expression host,...