Search
Search Results
-
Surface Glutamate Receptor Nanoscale Organization with Super-Resolution Microscopy (dSTORM)
Glutamate receptors mediate the majority of excitatory transmission in the central nervous system. Recent developments in super-resolution...
-
Quantitative Analysis of Single Glutamatergic Vesicles in the Brain
Amperometry is an electrochemical method that can provide quantitative as well as kinetic information on neurotransmitter release from single...
-
Chemogenetic Approaches to Study Astrocytes at Glutamatergic Synapses
Chemogenetic modulation, via the use of designer receptors exclusively activated by designer drugs (DREADDs), is an established in vivo approach that...
-
Alzheimer’s Disease Prevention and Treatment Based on Population-Based Approaches
The development of effective prevention and treatment strategies for Alzheimer’s disease (AD) and dementia is hindered by limited knowledge of the...
-
CSF N-Glycomics Using MALDI MS Techniques
In this chapter, we will present the methodology currently applied in our laboratory for the structural elucidation of the cerebrospinal fluid (CSF)...
-
CSF N-Glycomics Using High-Throughput UPLC-ESI Techniques in Alzheimer’s Disease
In this chapter, we will present a high-throughput method applied in our laboratory for the structural elucidation of the cerebrospinal fluid (CSF)...
-
In Silico Models to Validate Novel Blood-Based Biomarkers
Biological validation of preliminary findings is a key prerequisite in biomarker discovery. In recent years, the development of advanced large-scale...
-
Mass Spectrometry-Based Metabolomics Multi-platform for Alzheimer’s Disease Research
The integration of complementary analytical platforms is nowadays the most common strategy for comprehensive metabolomics analysis of complex...
-
High-Throughput Lipidomic and Metabolomic Profiling for Brain Tissue and Biofluid Samples in Neurodegenerative Disorders
Recent research has revealed the potential of lipidomics and metabolomics in identifying new biomarkers and mechanistic insights for...
-
Magnetic Resonance Spectroscopy (MRS) in Alzheimer’s Disease
MRS is a noninvasive technique to measure different metabolites in the brain. Changes in the levels of certain metabolites can be used as surrogate...
-
Analysis of Resting-State Functional Magnetic Resonance Imaging in Alzheimer’s Disease
Alzheimer’s disease (AD) has been characterized by widespread network disconnection among brain regions, widely overlap** with the hallmarks of the...
-
Analyses of Neural Circuits Governing Behavioral Plasticity in the Nematode Caenorhabditis elegans
Behavioral plasticity is subjected to various sensory stimuli, experiences, and physiological states, representing the temporal and spatial patterns...
-
Two-Photon FRET/FLIM Imaging of Cerebral Neurons
Two-photon FRET (Förster resonance energy transfer) and FLIM (fluorescence lifetime imaging microscopy) enable the detection of FRET changes of...
-
Immunohistochemistry of Brain Tissues
Immunohistochemistry (IHC) is the basis of histological or pathological analysis and is widely used to enable the detection and characterization of...
-
Genomic Copy Number Analysis Using Droplet Digital PCR: A Simple Method with EvaGreen Single-Color Fluorescent Design
Droplet digital PCR (ddPCR) is an emerging method for the absolute quantification of PCR products, and it can detect DNA copy numbers accurately. It...
-
Brain Sample Preparation After Performing in Utero Electroporation to Proliferating and Differentiated Cells in the Develo** Mouse Neocortex
In utero electroporation (IUE) enables labeling and manipulating specific types of cells by introducing DNA plasmids with desired promoters. After...
-
Social Behavior in Animal Models of Autism Spectrum Disorder
Autism spectrum disorder (ASD) is a complex neurodevelopmental disorder characterized by social deficits and stereotyped, repetitive patterns of...
-
Mouse Brain Tissue Preparation for Scanning Electron Microscopy
Scanning electron microscopy (SEM) is used to observe the surface structure of an object by irradiating an electron beam onto the sample and...
-
Single-Molecule FRET Analyses of NMDA Receptors
Single-molecule fluorescence resonance energy transfer (smFRET) enables the real-time observation of conformational changes in a single protein...
-
Functional Analysis of NMDAR Subunit Components in Postsynaptic Currents of Identified Cells and Synapses in Brain Slices
Epilepsy is one of the most represented neurological diseases worldwide. However, in many cases, the precise molecular mechanisms of epileptogenesis...