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Structures and Efflux Mechanisms of the AcrAB-TolC Pump
The global emergence of multidrug resistance (MDR) in gram-negative bacteria has become a matter of worldwide concern. MDR in these pathogens is...
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The Core Complex of Yeast COMPASS and Human Mixed-Lineage Leukemia (MLL), Structure, Function, and Recognition of the Nucleosome
Yeast COMPASS (complex of proteins associated with Set1) and human MLL (mixed-lineage leukemia) complexes are histone H3 lysine 4 methyltransferases...
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Human Transient Receptor Potential Ankyrin 1 Channel: Structure, Function, and Physiology
The transient receptor potential ion channel TRPA1 is a Ca2+-permeable nonselective cation channel widely expressed in sensory neurons, but also in...
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Structure and Function of the Glycosylphosphatidylinositol Transamidase, a Transmembrane Complex Catalyzing GPI Anchoring of Proteins
Glycosylphosphatidylinositol (GPI) anchoring of proteins is a ubiquitous posttranslational modification in eukaryotic cells. GPI-anchored proteins...
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Sample Preparation for Electron Cryo-Microscopy of Macromolecular Machines
High-resolution structure determination by electron cryo-microscopy underwent a step change in recent years. This now allows study of challenging...
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The Complementarity of Nuclear Magnetic Resonance and Native Mass Spectrometry in Probing Protein-Protein Interactions
Nuclear magnetic resonance (NMR) and native mass spectrometry (MS) are mature physicochemical techniques with long histories and important...
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Immunoprecipitation Methods to Isolate Messenger Ribonucleoprotein Complexes (mRNP)
Throughout their life cycle, messenger RNAs (mRNAs) associate with proteins to form ribonucleoproteins (mRNPs). Each mRNA is part of multiple...
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Peptide-Based Mass Spectrometry for the Investigation of Protein Complexes
In the last two decades, biological mass spectrometry has become the gold standard for the identification of proteins in biological samples. The...
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Principles of Tomographic Reconstruction
Imagine you need to look inside a living organism. You would have only two options: using invasive techniques or applying tomography. In this...
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After the Microscope: Long-Term Care of Electron Tomography Data
Cryogenic electron tomography (cryo-ET) produces datasets tremendously rich in information, with significant value beyond their originating study....
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Automated Cryo Electron Tomography Data Collection
Electron tomography entails the three-dimensional reconstruction of an object from its projections, which are acquired using a transmission electron...
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Beam-Induced Motion Mechanism and Correction for Improved Cryo-Electron Microscopy and Cryo-Electron Tomography
Beam induced motion (BIM) is a major cause of information loss in single-particle cryo-electron microscopy (cryo-EM) and cryo-electron tomography...
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Subtomogram Averaging
Subtomogram averaging (STA) is a method to determine macromolecular structures from electron tomograms, typically in their frozen-hydrated state...
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Electron Tomography of Cryo-Fixed and Resin-Embedded Samples
Room-temperature electron tomography of sections prepared from high-pressure frozen, freeze-substituted and resin-embedded cells is a powerful method...
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Metabolic Engineering for Improving Vegetable Oils
Synthetic biology’s application to metabolic engineering allows the production of biomolecules of interest for applications for the chemical,...
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The Paper Pulp Industry: From the Production of Cellulose Fibers to Second Generation Integrated Biorefinery
The paper pulp industry can be considered as the biggest actor in wood chemistry, the processes developped in its mills having been working as...
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Industrial Biotechnologies: A Hub at the Crossroads of the Food Industry and Green Chemistry
From ancient Egyptian brewing techniques to second-generation biorefineries, centuries of history look down upon us. But most of all, it is about...
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When THAT Rule Almost Persists as THAT Rule
In biology, theories are constantly enriched and adapted by new examples and scientific advances. In this chapter, we focus on the debate about the...
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Development of WB-EMS with Germany as an Blueprint for an Early Established Market
WB-EMS technology has its roots in Germany. Therefore, it is worthwhile to look at the German WB-EMS market to derive projections for the emergence...
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Evidence of WB-EMS on Different Outcomes
Health-related parameters are key outcomes of many WB-EMS trials (Le et al. 2024). In parallel, many studies focus on participants with diseases,...