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Article
Open AccessTopological N-glycosylation and site-specific N-glycan sulfation of influenza proteins in the highly expressed H1N1 candidate vaccines
The outbreak of a pandemic influenza H1N1 in 2009 required the rapid generation of high-yielding vaccines against the A/California/7/2009 virus, which were achieved by either addition or deletion of a glycosyl...
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Article
Open AccessAn autocrine inflammatory forward-feedback loop after chemotherapy withdrawal facilitates the repopulation of drug-resistant breast cancer cells
Stromal cells, infiltrating immune cells, paracrine factors and extracellular matrix have been extensively studied in cancers. However, autocrine factors produced by tumor cells and communications between auto...
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Article
Effects of Astragalus polysaccharides on antioxidant abilities and non-specific immune responses of Chinese mitten crab, Eriocheir sinensis
This study assessed the effects of Astragalus polysaccharides (APS) on antioxidant abilities, non-specific immune responses, and immune protective efficacy (attacked by Aeromonas hydrophila) of Eriocheir sinensis
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Article
Open AccessThe molecular determinants governing the immunogenicity of Japanese encephalitis live attenuated vaccines
In the course of isolating the attenuated Japanese encephalitis vaccine SA14-14-2, two attenuated strains SA14-9-7 and SA14-5-3 were also obtained that elicited low antibody responses in humans (<10% and 62%, ...
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Article
Open AccessImmunogenicity of Candidate MERS-CoV DNA Vaccines Based on the Spike Protein
MERS-coronavirus is a novel zoonotic pathogen which spread rapidly to >25 countries since 2012. Its apparent endemicity and the wide spread of its reservoir host (dromedary camels) in the Arabian Peninsula hig...
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Article
Open AccessDevelopment of in vitro and in vivo rabies virus neutralization assays based on a high-titer pseudovirus system
Pseudoviruses are useful virological tools because of their safety and versatility; however the low titer of these viruses substantially limits their wider applications. We developed a highly efficient pseudov...
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Article
Open AccessIntranasal Administration of Chitosan Against Influenza A (H7N9) Virus Infection in a Mouse Model
Influenza virus evolves constantly in an unpredictable fashion, making it necessary to vaccinate people annually for effective prevention and control of influenza. In general, however, during the first wave of...
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Chapter
Influence of High-Pressure Torsion on Mechanical Properties and Microstructural Evolution in 2197 Al-Li Alloy
2197 (Al-Li-Cu-Zr-Mn) alloy has been processed by High-pressure torsion (HPT) at applied pressure P of 2GPa and different shear strain γ in the range of 3 turns and 10 turns. Optical microscope (OM) and transm...
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Article
Response difference of transgenic and conventional rice (Oryza sativa) to nanoparticles (γFe2O3)
Nanoparticles (NPs) are an increasingly common contaminant in agro-environments, and their potential effect on genetically modified (GM) crops has been largely unexplored. GM crop exposure to NPs is likely to ...
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Article
Fate of 15N-enriched cyanobacteria feed for planktivorous fish in an enclosure experiment: a stable isotope tracer study
A tracer experiment using isotope 15N was performed to study the assimilation and retention of nitrogen from feces by two planktivorous fish, silver carp Hypophthalmichthys molitrix and tilapia Oreochromis niloti...
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Article
Open AccessPhytotoxic Mechanism of Nanoparticles: Destruction of Chloroplasts and Vascular Bundles and Alteration of Nutrient Absorption
This study focused on determining the phytotoxic mechanism of CeO2 nanoparticles (NPs): destroying chloroplasts and vascular bundles and altering absorption of nutrients on conventional and Bt-transgenic cottons....
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Article
Open AccessUptake, transport, distribution and Bio-effects of SiO2 nanoparticles in Bt-transgenic cotton
SiO2 nanoparticle is one of the most popular nanomaterial which has been used in various fields, such as wastewater treatment, environmental remediation, food processing, industrial and household applications, bi...
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Article
Open AccessA universal monoclonal antibody protects against all influenza A and B viruses by targeting a highly conserved epitope in the viral neuraminidase
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Article
Rosuvastatin attenuates the elevation in blood pressure induced by overexpression of human C-reactive protein
C-reactive protein (CRP) has been shown to function as an inflammatory factor to induce endothelial dysfunction and hypertension in rats. The anti-inflammatory effects of statins suggest that they may attenuat...
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Article
O-Linked Glycosylation Leads to Decreased Thermal Stability of Interferon Alpha 2b as Measured by Two Orthogonal Techniques
Thermal stability is considered an indication of protein fold and conformational stability. We investigate the influence of glycosylation on the thermal stability of interferon alpha 2b (IFN α-2b).
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Article
A simple slot blot for the detection of virtually all subtypes of the influenza A viral hemagglutinins using universal antibodies targeting the fusion peptide
The fusion peptide of influenza virus hemagglutinin (HA) has a critical role in mediating the entry of the virus into the cells and is also the only universally conserved sequence in the HAs of all strains of ...
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Article
The interaction between thymine DNA glycosylase and nuclear receptor coactivator 3 is required for the transcriptional activation of nuclear hormone receptors
The T:G mismatch specific DNA glycosylase (TDG) is known as an important enzyme in repairing damaged DNA. Recent studies also showed that TDG interacts with a p160 protein, steroid receptor coactivator 1 or nu...
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Protocol
Mammalian Two-Hybrid Assay for Detecting Protein-Protein Interactions in Vivo
The mammalian two-hybrid system is a very powerful tool to investigate protein-protein interactions in terms of functional domains and identify potential binding ligands and partners of a protein. Compared wit...
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Article
Single-chain antibody-mediated gene delivery into ErbB2-positive human breast cancer cells
Targeted gene transfer by nonviral vectors can be achieved through incorporation of specific ligand(s) into the vectors. In this study, the effects of incorporation of an anti-ErbB2 single-chain antibody fragm...
