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Article
Cloning of 9-cis-epoxycarotenoid dioxygenase gene (TaNCED1) from wheat and its heterologous expression in tobacco
Abscisic acid (ABA) regulates plant responses to various environmental stresses. Oxidative cleavage of cis-epoxycarotenoids catalyzed by 9-cis-epoxycarotenoid dioxygenase (NCED) is the critical step in the biosyn...
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Article
A type I MADS-box gene is differentially expressed in wheat in response to infection by the stripe rust fungus
The gene, designated TaMADS2, was obtained from wheat leaves infected with the wheat stripe rust fungus by in silico cloning and RT-PCR. TaMADS2 encodes a predicted 159-amino-acid polypeptide that contains a high...
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Article
Doubled haploids of novel trigenomic Brassica derived from various interspecific crosses
To develop doubled haploid (DH) map** populations of hexaploid Brassica, 10 F1 hybrids derived from crosses between allohexaploid Brassica parents were evaluated in this study. The allohexaploid Brassica parent...
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Article
Amelioration of salt-induced oxidative stress in eggplant by application of 24-epibrassinolide
The effects of exogenous 24-epibrassinolide (EBR) on the growth, oxidative damage, antioxidant system and ion contents in eggplant (Solanum melongena L.) seedlings under salt stress were investigated. Eggplant se...
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Article
Differential proteomic analysis of cadmium-responsive proteins in wheat leaves
To gain a comprehensive understanding of plant response to Cd, physiological and proteomic changes in wheat (Triticum aestivum L.) leaves exposed to a range of Cd concentrations (10, 100 and 200 μM) were investig...
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Article
Effects of different conservation methods on the genetic stability of potato germplasm
A total of 50 potato (Solanum tuberosum L.) varieties were maintained in vitro or under field conditions at Keshan National Genebank (KNG) for 15 years. At the end of the conservation period, a two-year field tri...
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Article
QTL detected for grain-filling rate in maize using a RIL population
The grain-filling rate plays an important role in determining grain yield. To elucidate the genetic basis of the grain-filling rate, a set of 203 recombinant inbred lines was evaluated at two locations over 2 ...
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Article
Rapid screening of Musa species for resistance to Fusarium wilt in an in vitro bioassay
In order to accelerate breeding and selection for disease resistance to Fusarium wilt, it is important to develop bioassays which can differentiate between resistant and susceptible cultivars efficiently. Curr...
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Article
Silicon Improves the Tolerance to Water-Deficit Stress Induced by Polyethylene Glycol in Wheat (Triticum aestivum L.) Seedlings
Drought stress usually causes a serious yield reduction in wheat production. Silicon (Si) has been reported to be able to alleviate drought stress damage; however, the mechanism is still poorly understood. In ...
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Article
Intertribal somatic hybrids between Brassica napus and Camelina sativa with high linolenic acid content
Intertribal somatic hybrids of Brassica napus and Camelina sativa were developed by protoplast electrofusion. Hybrid identity of the regenerants was determined using flow cytometric analysis of nuclear DNA conten...
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Article
The involvement of an expansin geneTaEXPB23 from wheat in regulating plant cell growth
Expansins, found in the cell wall, have the unique ability to induce immediate cell wall extension. In this study, a β-expansin gene (TaEXPB23) isolated from wheat (Triticum aestivum L.) coleoptiles was transform...
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Article
The role of salicylic acid in response of two rice cultivars to chilling stress
Two rice (Oryza sativa L.) cultivars differing in chilling sensitivity, Changbaijiu (chilling-tolerant) and Zhongjian (chilling-sensitive) were pre-treated with 0.5, 1.0 and 2.0 mM salicylic acid (SA) for 24 h be...
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Article
Determination of oxalate in plant tissues with oxalate oxidase prepared from wheat
A method for determination of oxalate with oxalate oxidase (OxO, EC 1.2.3.4) prepared from wheat bran, is based on specific oxidation of oxalate to produce H2O2. The H2O2 formed was colorimetrically determined us...
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Article
Characterization of a rice metallothionein type 3 gene with different expression profiles under various nitrogen forms
A cDNA sequence OsMT3 was initially isolated from the subtractive cDNA library of ammonium-fed rice (Oryza sativa L.) leaves, which was further confirmed by Northern blot to be highly ammonium-up-regulated as com...
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Article
A novel bioassay method: Determining bioactivity of compounds against rice blast (Magnaporthe grisea) with barley plants
This paper developed an in vivo bioassay method of determining bioactivity of compounds against Magnaporthe grisea (Hebert) Barr. with barley (Hordeum vulgare L.) plants, instead of rice (Oryza sativa L.) plants....
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Article
Effect of glycinebetaine on function of thylakoid membranes in wheat flag leaves under drought stress
Two wheat cultivars, HF9703 (drought tolerant) and SN215953 (drought sensitive) were used to examine the effects of glycinebetaine (GB, 100 mM) on lipid composition and function of thylakoid membranes under dr...
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Article
High efficient transgenic plant regeneration from embryogenic calluses of Citrus sinensis
Transformation and high efficient regeneration of transgenic plants from embryogenic calluses of Bingtang sweet orange [Citrus sinensis (L.) Osbeck] was reported. Embryogenic calluses were inoculated with Agrobac...
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Chapter and Conference Paper
Protoplast Technology and Citrus Improvement
Citrus is one most important fruit crop in China and worldwide. Protoplast fusion has been an effective and successful technique for citrus improvement by circumventing reproductive barriers such a...
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Article
Isolation of cytoplasts from Satsuma mandarin (Citrus unshiu Marc.) and production of alloplasmic hybrid calluses via cytoplast–protoplast fusion
Cytoplasm of Satsuma mandarin (Citrus unshiu Marc.) is known to influence seedlessness. Transfer of cytoplasm to a seedy cultivar could possibly lead to the production of seedless citrus fruits. In the present pa...
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Article
Dynamics of zinc uptake and accumulation in the hyperaccumulating and non-hyperaccumulating ecotypes of Sedum alfredii Hance
Sedum alfredii Hance has been identified as a Zn-hyperaccumulating plant species native to China. The characteristics of Zn uptake and accumulation in the hyperaccumulating ecotype (HE) and non-hyperaccumulating ...