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Article
Open AccessThe structural basis of an NADP+-independent dithiol oxidase in FK228 biosynthesis
The disulfide bond is unusual in natural products and critical for thermal stability, cell permeability and bioactivity. DepH from Chromobacterium violaceum No. 968 is an FAD-dependent enzyme responsible for cata...
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Article
Enhanced enantioselectivity of a carboxyl esterase from Rhodobacter sphaeroides by directed evolution
The present work created an esterase variant from Rhodobacter sphaeroides (RspE) with enhanced selectivity in hydrolytic kinetic resolutions by directed evolution. A “model” substrate, methyl mandelate, was intro...
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Article
Solution structure of the Magnaporthe oryzae avirulence protein AvrPiz-t
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Article
Open AccessThe mutation network for the hemagglutinin gene from the novel influenza A (H1N1) virus
A mutation network for the hemagglutinin gene (HA) of the novel type A (H1N1) influenza virus was constructed. Sequence homology analysis indicated that one HA sequence type from the viruses mainly isolated from ...
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Article
Open AccessSubty** of type A influenza by sequencing the variable regions of HA gene specifically amplified with RT-PCR
The outbreak of a novel influenza A (H1N1) virus across the globe poses a threat to human health. It is of paramount importance to develop a rapid, reliable and inexpensive diagnostic procedure. Based on the b...
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Article
Open AccessStructure modeling and spatial epitope analysis for HA protein of the novel H1N1 influenza virus
In recent months, a novel influenza virus H1N1 broke out around the world. With bioinformatics technology, the 3D structure of HA protein was obtained, and the epitope residues were predicted with the method d...
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Article
Structural determinants of SecB recognition by SecA in bacterial protein translocation
SecB is a bacterial chaperone involved in directing pre-protein to the translocation pathway by its specific interaction with the peripheral membrane ATPase SecA. The SecB-binding site on SecA is located at it...
