1,647 Result(s)
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Article
Establishment and characterization of a continuous murine uterine cervix cancer cell line metastatic to lymph nodes and lungs
The murine uterine cervix cancer (MUCC) cell line was derived from a chemically induced Kunming mouse uterine cervix cancer (U27) and maintained in culture on solid substrates for over 100 passages. Cultures w...
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Article
Spontaneous acquisition of tumorigenicity and invasiveness by mouse lens explant cells during culture in vitro
The lens of the eye is one of the rare organs in which tumors do not occur spontaneously. It therefore appeared to us that lens cells would not present the background of spontaneous transformation toward malig...
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Role of interspecies transfer of chromosomal genes in the evolution of penicillin resistance in pathogenic and commensal Neisseria species
The two pathogenic species of Neisseria, N. meningitidis and N. gonorrhoeae, have evolved resistance to penicillin by alterations in chromosomal genes encoding the high molecular weight penicillin-binding protein...
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Article
Map** of the human integrin β7 gene (ITGβ7) to 12q13.13 by non-isotopic in situ hybridization
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Article
A DNA-binding protein factor in K562 nuclear extract interacts with positive control region (PCR) in the 5′-flanking sequence of human β-globin gene
It has been known that there are at least three regulatory regions (NCR1, NCR2 and PCR) in the 5′-flanking sequence (from −610 bp to +1 bp) of human β-globin gene and that the function of PCR is unique to the hum...
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Article
Proteins binding to the 5′-flanking regulatory elements of the human β-globin gene
The binding of nuclear proteins prepared from mouse erythroid tissue in different developmental stages to the 5′-flanking regulatory elements of human β-globin gene, two negative control regions(NCR1, -610 to -49...
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Article
Identification of the development stage-specific factors in mouse fetal liver binding to the human β-globin gene promoter
In order to elucidate the molecular mechanisms of globin gene expression during embryonic development, the nuclear extracts from mouse hematopoietic tissue at different stages of development have been prepared...
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Article
A stage-specific protein factor binding to a CACCC motif in both human β-globin gene promoter and 5'-HS2 region
The DNaseI hypersensitive site 2 (HS2) of human β- globin locus control region (LCR) is required for the high level expression of human β-globin genes. In the present study, a stage-specific protein factor (LPF-β
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Article
Construction of Oryza sativa genome contigs by finger print strategy
We described the construction of BAC contigs of the genome of a indica variety of Oryza sativa, Guang Lu Ai 4. An entire representative (sixfold coverage of rice chromosomes) and genetically stable BAC library of...
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Article
Changes of macromolecular organizations in nonjunctional sarcolemmas after cross-innervation— a study of fast-and slow-twitch muscle fibres in rats
The purpose of the present study was to analyse the changes of macromolecular organizations in nonjunctional sarcolemmas of different types of skeletal muscle fibres after cross-innervation. In normal rats the...
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Article
An in vivo system for analysis of stable complex formation between Src and AFAP-110
The actin filament-associated protein, AFAP-110, forms a stable complex with activated pp60src (Src) as both an SH2 and SH3 binding partner. AFAP-110 contains two putative SH3 binding motifs and six putative SH2 ...
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Article
Studies on DNA-protein interactions in the upstream regulatory region of the human ɛ-globin gene promoter
The erythroid- and developmental stage-specific expression of the human ɛ-globin gene is controlled, in part, by the 5′-flanking DNA sequence of this gene. In the present study, we have used DNA-protein bindin...
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Article
Quantitative study of organelle nucleoids during the second pollen grain mitosis inOenothera biennis
The behavior of organelle nucleoids in the generative cell was examined at the second (pollen grain) mitosis by epifluorescence microscopy after staining with 4′,6-diamidino-2-phenylindole (DAPI) inOenothera bien...
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Article
The apoptosis of HEL cells induced by hydroxyurea
Hydroxyurea has been used to synchronize cultured cells to S-phase and used to treat patients with sickle-cell anemia. Recently, we found that hydroxyurea can induce the apoptosis of HEL (human erythroleukemia...
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Article
Visualization of chromatin folding patterns in chicken erythrocytes by atomic force microscopy (AFM)
The organization of the higher order structure of chromatin in chicken erythrocytes has been examined with tap**-mode scanning force microscopy under conditions close to their native environment. Reproducibl...
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Article
Trans-acting factors from the human fetal liver binding to the human ɛ-globin gene silencer
The developmental stage-specific silencing of the human ɛ-globin gene during embryonic life is controlled, in part, by the silencer (−392bp ∼ −177bp) upstream of this gene. In order to elucidate its role, the nuc...
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Article
Characterization of troponin-C interactions in skinned barnacle muscle: comparison with troponin-C from rabbit striated muscle
Previously it was shown that when troponin-C (TnC) is extracted from barnacle myofibrillar bundles they lose their Ca2+ sensitivity, which can be restored by adding back barnacle TnC (either isoform, BTnC1 or ...
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Article
Plant regeneration from in vitro-cultured seedling leaf protoplasts of Actinidia eriantha Benth
Newly expanded in vitro leaves of Actinidia eriantha were used for protoplast isolation. Protoplasts were cultured in liquid MS medium (lacking NH4NO3) supplemented with 2,4-D (2,4-dichlorophenoxyacetic acid) an...
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Article
The 5′-flanking cis-acting elements of the human ɛ-globin gene associates with the nuclear matrix and binds to the nuclear matrix proteins
The nuclear matrix attachment regions(MARs) and the binding nuclear matrix proteins in the 5′-flanking cis acting elements of the human ɛ-globin gene have been examined. Using in vitro DNA-matrix binding assay...
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Differential induction of apoplastic peroxidase and chitinase activities in susceptible and resistant wheat cultivars by Russian wheat aphid infestation
The intercellular peroxidase and chitinase activities of three wheat cultivars [Triticum aestivum L. cvs `Tugela DN', `Molopo DN' (Gariep) and `Betta DN'] containing the Dn-1 gene for resistance to the Russian w...
