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Immunohistochemical Detection of Sphingosine-1-Phosphate and Sphingosine Kinase-1 in Human Tissue Samples

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Sphingosine-1-Phosphate

Part of the book series: Methods in Molecular Biology ((MIMB,volume 874))

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Abstract

Sphingosine-1-phosphate (S1P) and the enzyme primarily responsible for its production, sphingosine kinase-1 (SphK-1), are thought to be dysregulated in multiple human diseases including cancer, multiple sclerosis (MS), diabetes, neurological diseases, fibrosis, and certain pathologies associated with impaired angiogenesis such as, age-related macular degeneration (AMD). Antibody-based techniques to identify and localize S1P and SphK-1 within cells and tissue specimens represent powerful tools not only to understand the biological role of these molecules but also to validate these unique in-class targets in multiple state diseases. Consequently, the potential applications of these molecules for therapy and diagnostic purposes are currently under investigation. Here, we describe two staining procedures for identification of S1P and SphK-1 in human frozen tissue samples and the challenges encountered in the process of localization in tissue samples of lipid molecules, such as S1P.

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Author information

Authors and Affiliations

  1. Lpath Incorporated, San Diego, CA, USA

    Barbara Visentin & Roger Sabbadini

  2. Centre for Liver Research, NIHR Biomedical Research Unit, University of Birmingham & Queen Elizabeth Hospital Birmingham NHS Trust, Birmingham, UK

    Gary Reynolds

Authors
  1. Barbara Visentin
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  2. Gary Reynolds
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  3. Roger Sabbadini
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Corresponding author

Correspondence to Barbara Visentin .

Editor information

Editors and Affiliations

  1. University of Melbourne, Parkville, Victoria, Australia

    Alice Pébay

  2. Sprott Centre for Stem Cell Research, Regenerative Medicine Program, Ottawa Hospital Research Institute, Smyth Road 501, Ottawa, K1Y 8L6, Canada

    Kursad Turksen

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Visentin, B., Reynolds, G., Sabbadini, R. (2012). Immunohistochemical Detection of Sphingosine-1-Phosphate and Sphingosine Kinase-1 in Human Tissue Samples. In: Pébay, A., Turksen, K. (eds) Sphingosine-1-Phosphate. Methods in Molecular Biology, vol 874. Humana Press. https://doi.org/10.1007/978-1-61779-800-9_5

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  • DOI: https://doi.org/10.1007/978-1-61779-800-9_5

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-799-6

  • Online ISBN: 978-1-61779-800-9

  • eBook Packages: Springer Protocols

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