Abstract
An important step in the analysis of protein function is identification of the interaction partners of each protein. The two-hybrid system has been widely used to identify and explore protein-protein interactions. By using various two-hybrid systems, numerous protein interactions that regulate apoptosis signaling have been discovered that reveal unexpected functions of cancer-relevant proteins. Methods for performing two-hybrid experiments using either yeast or mammalian cells will be described in this chapter.
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Matsuzawa, Si., Reed, J.C. (2007). Yeast and Mammalian Two-Hybrid Systems for Studying Protein-Protein Interactions. In: Fisher, P.B. (eds) Cancer Genomics and Proteomics. Methods in Molecularbiologyâ„¢, vol 383. Humana Press. https://doi.org/10.1007/978-1-59745-335-6_14
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DOI: https://doi.org/10.1007/978-1-59745-335-6_14
Publisher Name: Humana Press
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