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SRYTH: A New Yeast Two-Hybrid Method

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Candida Species

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1356))

Abstract

Many biological processes are regulated by protein-protein interactions, and the analysis of these interactions has been a productive endeavor contributing to our understanding of cellular organization and function. The yeast two-hybrid technique is a widely used, powerful method of analyzing protein-protein interactions. The currently used formats, however, have inherent limitations, providing an opportunity to develop new alternatives that extend our ability to detect protein-protein interactions of biological relevance. Here we present a two-hybrid system named SRYTH (Ste11p/Ste50p related yeast two-hybrid) based on the Ste11p/Ste50p interaction that uses the activation of the HOG pathway of Saccharomyces cerevisiae as a reporter for interactions. The system is suitable for detecting cytoplasmic protein interactions in their natural subcellular environment, and has been successfully used to investigate protein-protein interactions, including transcription factor associations, in Candida albicans.

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Author information

Authors and Affiliations

  1. Biology Department, Concordia University, 7141 Sherbrooke St. West, Montreal, QC, Canada, H4B 1R6

    Jaideep Mallick & Malcolm Whiteway

  2. Biochemistry Department, McGill University, 3655 Promenade Sir William Osler, Montreal, QC, Canada, H3G 1Y6

    Gregor Jansen

  3. National Research Council of Canada, 6100 Royalmount Ave, Montreal, QC, Canada, H4P 2R2

    Cunle Wu

  4. Division of Experimental Medicine, McGill University, 1100 Pine Ave.West, Montreal, QC, Canada, H3A 1A3

    Cunle Wu

Authors
  1. Jaideep Mallick
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  2. Gregor Jansen
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  3. Cunle Wu
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  4. Malcolm Whiteway
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Corresponding author

Correspondence to Malcolm Whiteway .

Editor information

Editors and Affiliations

  1. Dept Microbiology & Immunology, Georgetown University Medical Center, Washington, District of Columbia, USA

    Richard Calderone

  2. Dept of Microbiology & Immunology, Georgetown University Medical Center, Washington, District of Columbia, USA

    Ronald Cihlar

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© 2016 Springer Science+Business Media New York

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Mallick, J., Jansen, G., Wu, C., Whiteway, M. (2016). SRYTH: A New Yeast Two-Hybrid Method. In: Calderone, R., Cihlar, R. (eds) Candida Species. Methods in Molecular Biology, vol 1356. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3052-4_3

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  • DOI: https://doi.org/10.1007/978-1-4939-3052-4_3

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3051-7

  • Online ISBN: 978-1-4939-3052-4

  • eBook Packages: Springer Protocols

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