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Flow Cytometry-Based Assessment of Antibody Function Against Malaria Pre-erythrocytic Infection

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Malaria Vaccines

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1325))

Abstract

The development of new interventional strategies against pre-erythrocytic malaria is hampered by the lack of standardized approaches to assess inhibition of sporozoite infection of hepatocytes. The following methodology, based on flow cytometry, can be used to quantitatively assess P. falciparum sporozoite infection in vitro in medium throughput. In addition to assessing the efficacy of antibodies, this assay has a wide variety of applications for investigating basic science questions about the malaria liver stage. This approach is easily applied in a variety of laboratory settings, assesses the functionality of antibody responses against malaria sporozoites, and can be adapted for the limited quantities of sample which are typically available from clinical investigations.

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Authors and Affiliations

  1. Center for Infectious Disease Research, 307 Westlake Ave. N, Suite 500, Seattle, WA, 98109, USA

    Alyse N. Douglass, Peter G. Metzger, Stefan H. I. Kappe & Alexis Kaushansky

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  1. Alyse N. Douglass
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  2. Peter G. Metzger
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  3. Stefan H. I. Kappe
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  4. Alexis Kaushansky
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Correspondence to Alexis Kaushansky .

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  1. Center for Infectious Disease Research, Seattle, Washington, USA

    Ashley Vaughan

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Douglass, A.N., Metzger, P.G., Kappe, S.H.I., Kaushansky, A. (2015). Flow Cytometry-Based Assessment of Antibody Function Against Malaria Pre-erythrocytic Infection. In: Vaughan, A. (eds) Malaria Vaccines. Methods in Molecular Biology, vol 1325. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2815-6_4

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  • DOI: https://doi.org/10.1007/978-1-4939-2815-6_4

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2814-9

  • Online ISBN: 978-1-4939-2815-6

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